Team:Sevilla/Week10
From 2011.igem.org
Inesgarcia (Talk | contribs) |
Elena moreno (Talk | contribs) |
||
Line 165: | Line 165: | ||
<p>What the...FUCK? Honestly,is somebody kidding us? Where's the hidden camera? What the hells' wrong? Not a single colony in our transformation plates. AGAIN. Will it EVER work, for goodness sake? We've tried almost everything! Should we pray to God, to Superman, to Mr. Gibss?? Let's start again... :( </p> | <p>What the...FUCK? Honestly,is somebody kidding us? Where's the hidden camera? What the hells' wrong? Not a single colony in our transformation plates. AGAIN. Will it EVER work, for goodness sake? We've tried almost everything! Should we pray to God, to Superman, to Mr. Gibss?? Let's start again... :( </p> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</div> | </div> | ||
Line 202: | Line 183: | ||
</p> | </p> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</div> | </div> | ||
Line 271: | Line 234: | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</div> | </div> | ||
Line 306: | Line 251: | ||
</p> | </p> | ||
<p>On the other hand, our constructions haven't arrived on time so we'll have to give up that option and stick to what we have now. We're having a bussy weekend: the parts must be sent next week and there's still a lot to do.LAST PUSH GUYS! | <p>On the other hand, our constructions haven't arrived on time so we'll have to give up that option and stick to what we have now. We're having a bussy weekend: the parts must be sent next week and there's still a lot to do.LAST PUSH GUYS! | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</p> | </p> | ||
- | |||
</div> | </div> | ||
Line 339: | Line 266: | ||
<div class="summary"> | <div class="summary"> | ||
- | <p>Marathon weekend doing mini-preps of every single colony we have, with their restrictions, electrophoresis, purification, etc. This means more than 30 digestions ONLY for one construction. Besides, we're also working with some of the other planned constructions, and we're doing everything in duplicate to try different protocols in line. We're running out of time,and the electrophoresis machinery is smoking. We're using two different competent cells, kipping the | + | <p>Marathon weekend doing mini-preps of every single colony we have, with their restrictions, electrophoresis, purification, etc. This means more than 30 digestions ONLY for one construction. Besides, we're also working with some of the other planned constructions, and we're doing everything in duplicate to try different protocols in line. We're running out of time,and the electrophoresis machinery is smoking. We're using two different competent cells, kipping the ligations at different temperatures at different times, amplifying small fragments by pcr...We have to try everything! |
</p> | </p> | ||
<p> We're taking turns to get some sleep, but the floor is not exactly comfortable, and the cleaners come at 6 a.m... At least we have enough food to survive, a coffee machine and 4 litres of Coca-Cola. What a long weekend...</p> | <p> We're taking turns to get some sleep, but the floor is not exactly comfortable, and the cleaners come at 6 a.m... At least we have enough food to survive, a coffee machine and 4 litres of Coca-Cola. What a long weekend...</p> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
</html> | </html> |
Revision as of 18:03, 21 September 2011
Monday 12 September
One more week. We get to the lab in the morning. We open the incubator...
What the...FUCK? Honestly,is somebody kidding us? Where's the hidden camera? What the hells' wrong? Not a single colony in our transformation plates. AGAIN. Will it EVER work, for goodness sake? We've tried almost everything! Should we pray to God, to Superman, to Mr. Gibss?? Let's start again... :(
Tuesday 13 September
At least the boys are coming back little by little and we have more hands in the lab to work, and this is a bit more crowded and cheerful. We're starting to think more seriously about the Jamboree, there's still so much to do about the wiki, the parts, the team t-shirts, the flights, etc.
Wednesday 14 September
While we keep fighting against our naughty bacteria and the "standard" parts, we're also testing the biobrick T9002, developed by the Cambridge Lab in 2005. Ana is helping us to plan the experiments.
Wednesday Protocols
IMPROVED PROTOCOLS
Ligation
We are going to bind DNA small fragments with bigger plasmids, so we will use more nt of fragments
Thursday 15 September
Ligation 1 has grown!!!! Isn't it incredible? It's a fusion of F2620 and I732019, it should be able to sense C6-3-oxo-HSL in the media and express lacZ as a reporter. We'll have to make a liquid culture to get the plasmid tomorrow, digest it to check if the insert has the appropiate length and see if it works. For the last part, we seed the bacteria in a plate with the signal and XGal that should turn blue when lacZ is expressed.
Friday 16 September
YEAAAAAAAAAH! IT WORKS!!! Look at this plate and the little blue spots! IT WORKS! Sadly,we have to digest the plasmid, extract the insert and place it in pSB1C3, because now it's in pSB1A2. Let's hope this time the cycle works as it should.
On the other hand, our constructions haven't arrived on time so we'll have to give up that option and stick to what we have now. We're having a bussy weekend: the parts must be sent next week and there's still a lot to do.LAST PUSH GUYS!
Weekend
Marathon weekend doing mini-preps of every single colony we have, with their restrictions, electrophoresis, purification, etc. This means more than 30 digestions ONLY for one construction. Besides, we're also working with some of the other planned constructions, and we're doing everything in duplicate to try different protocols in line. We're running out of time,and the electrophoresis machinery is smoking. We're using two different competent cells, kipping the ligations at different temperatures at different times, amplifying small fragments by pcr...We have to try everything!
We're taking turns to get some sleep, but the floor is not exactly comfortable, and the cleaners come at 6 a.m... At least we have enough food to survive, a coffee machine and 4 litres of Coca-Cola. What a long weekend...