Team:EPF-Lausanne/Our Project/TetR mutants/muTetRs
From 2011.igem.org
(Difference between revisions)
(→What) |
(→What) |
||
Line 22: | Line 22: | ||
| <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36F</a> </html> ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613013|||| | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36F</a> </html> ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613013|||| | ||
|- | |- | ||
- | | V36FW43S||PCR-induced mutagenesis||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014|||| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">V36FW43S</a> </html> ||PCR-induced mutagenesis||T7||His-tag||linear ||(ins C-term), ITT tested||||BBa_K613014|||| |
|- | |- | ||
| |||||||||||||||||| | | |||||||||||||||||| | ||
|- | |- | ||
- | | E37AW43ST141A||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">E37AW43ST141A</a> ||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v |
|- | |- | ||
| |||||||||||||||||| | | |||||||||||||||||| | ||
Line 32: | Line 32: | ||
| |||||||||||||||||| | | |||||||||||||||||| | ||
|- | |- | ||
- | | P39K||PCR-induced mutagenesis||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">P39K</a> ||PCR-induced mutagenesis||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v |
|- | |- | ||
| |||||||||||||||||| | | |||||||||||||||||| | ||
|- | |- | ||
- | | Y42F||PCR-induced mutagenesis||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">Y42F</a>||PCR-induced mutagenesis||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v |
|- | |- | ||
- | | Y42FK108E||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">Y42FK108E</a>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v |
|- | |- | ||
| |||||||||||||||||| | | |||||||||||||||||| | ||
|- | |- | ||
- | | P39QY42M ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613019|||| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">P39QY42M</a> ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613019|||| |
|- | |- | ||
- | | P39QY42ML197S||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613020|||| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">P39QY42ML197S</a>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613020|||| |
|- | |- | ||
- | | P39QY42ML52P||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613021|||| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">P39QY42ML52P</a>||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613021|||| |
|- | |- | ||
| |||||||||||||||||| | | |||||||||||||||||| | ||
|- | |- | ||
- | | E37AP39K||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613022||v|| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">E37AP39K</a>||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613022||v|| |
|- | |- | ||
| |||||||||||||||||| | | |||||||||||||||||| | ||
|- | |- | ||
- | | E37AP39QY42F||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v|| | + | | <html> <a href="http://partsregistry.org/Part:BBa_K613013">E37AP39QY42F</a>||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v|| |
|} | |} | ||
{{:Team:EPF-Lausanne/Templates/Footer}} | {{:Team:EPF-Lausanne/Templates/Footer}} |
Revision as of 16:47, 21 September 2011
muTetRs
In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outlineWhy
How
What
TetR variants | Method | Promoter | C-terminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
V36F | site directed mutagenesis | SP6 | eGFP | plasmid | worked well | v | BBa_K613013 | ||
V36FW43S | PCR-induced mutagenesis | T7 | His-tag | linear | (ins C-term), ITT tested | BBa_K613014 | |||
E37AW43ST141A ||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v |- | |||||||||||||||||| |- | |||||||||||||||||| |- | P39K ||PCR-induced mutagenesis||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v |- | |||||||||||||||||| |- | Y42F||PCR-induced mutagenesis||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v |- | Y42FK108E||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v |- | |||||||||||||||||| |- | P39QY42M ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613019|||| |- | P39QY42ML197S||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613020|||| |- | P39QY42ML52P||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613021|||| |- | |||||||||||||||||| |- | E37AP39K||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613022||v|| |- | |||||||||||||||||| |- | E37AP39QY42F||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v|| |} {{:Team:EPF-Lausanne/Templates/Footer}} |