Revision as of 16:41, 21 September 2011

muTetRs

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

TetR variants	Method	Promoter	C-terminal	DNA form	Expression	MITOMI vs 1-off	Biobrick	Sequenced	Sent to registry	in vivo test

V36F	site directed mutagenesis	SP6	eGFP	plasmid	worked well	v	BBa_K613013			might not bind ATC
V36FW43S	PCR-induced mutagenesis	T7	His-tag	linear	(ins C-term), ITT tested		BBa_K613014			might not bind ATC

E37AW43ST141A	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested	v	BBa_K613015	v	v	might not bind ATC


P39K	PCR-induced mutagenesis	T7	His-tag	template	ITT tested and worked ok	v	BBa_K613016	v	v	failed (Gibson?) will be sequenced

Y42F	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested	v	BBa_K613017	v	v	Gibson failed
Y42FK108E	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested		BBa_K613018	v	v	behaves as WT

P39QY42M	site directed mutagenesis	SP6	eGFP	plasmid	worked well	v	BBa_K613019			failed (Gibson?) will be sequenced
P39QY42ML197S	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested		BBa_K613020
P39QY42ML52P	PCR-induced mutagenesis	T7	His-tag	linear	ITT tested		BBa_K613021

E37AP39K	site directed mutagenesis	T7	His-tag	linear			BBa_K613022	v

E37AP39QY42F	site directed mutagenesis	T7	His-tag	linear			BBa_K613023	v

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 ==What==
-{| font="40" {{table}}
+{| {{table}}  style="font-size: 60%;"
 | align="center" style="background:#f0f0f0;"|'''TetR variants'''
 | align="center" style="background:#f0f0f0;"|'''Method'''