Team:UT-Tokyo/Data/Method

From 2011.igem.org

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{{:Team:UT-Tokyo/Templates/BeginContent|fullpagename=Team:UT-Tokyo/Project/Method|subpagename=Method}}
{{:Team:UT-Tokyo/Templates/BeginContent|fullpagename=Team:UT-Tokyo/Project/Method|subpagename=Method}}
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<!-- 海老沼です。ちょい編集してみました。html、cssも使えるんですねぇ。なんか間違っていたらコメントください。
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<!-- 海老沼です。ちょい編集してみました。html、cssも使えるんですねぇ。
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しかしテーブルのスタイル設定が手間です。cssでどっかに書いてしまうものなのだろうかw
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とりあえず、最初の列以外はtext-align:rightでいくと見栄えがいい感じなんですが。
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↓ついいじったのが久しぶりでノリで2カラムレイアウトにしてみました。苦言が呈され次第削除します…
↓ついいじったのが久しぶりでノリで2カラムレイアウトにしてみました。苦言が呈され次第削除します…
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Before use, add 10ml Mg sol.
Before use, add 10ml Mg sol.
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Mg sol.
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:Mg sol.
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{|
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:{|
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!align="left" style="width:200px"|reagents
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!align="left" style="width:150px"|reagents
!align="right" style="width:80px"|final conc.
!align="right" style="width:80px"|final conc.
!align="right" style="width:80px"|amount
!align="right" style="width:80px"|amount
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*M9
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*20&times; M9 medium
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{|
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!align="left" style="width:200px"|reagents
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!align="right" style="width:80px"|final conc.
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!align="right" style="width:80px"|amount
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|-
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|Na<span class="sub">2</span>HPO<span class="sub">4</span>
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|align="right"|-
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|align="right"|6.0g
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|-
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|KH<span class="sub">2</span>PO<span class="sub">4</span>
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|align="right"|-
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|align="right"|3.0g
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|-
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|NaCL
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|align="right"|-
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|align="right"|0.5g
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|-
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|NH<span class="sub">4</span>Cl
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|align="right"|-
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|align="right"|1.0g
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|-
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|style="border-bottom: 2px solid"|MilliQ
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|style="text-align:center; border-bottom: 2px solid"|-
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|style="text-align:right; border-bottom: 2px solid"|50ml
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|-
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|colspan="2"|Total
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|style="text-align:right"|50ml
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|}
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After A.C. , add following reagents to 1L M9 medium
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:{|
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!align="left" style="width:150px"|reagents
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!align="right" style="width:80px"|final conc.
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!align="right" style="width:80px"|amount
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|-
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|1M MgSO<span clas="sub">4</span>
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|align="right"|-
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|align="right"|1.0ml
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|-
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|2M Glucose
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|align="right"|-
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|align="right"|5.6ml
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|-
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|1% Thiamine
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|align="right"|-
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|align="right"|1.0ml
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|-
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|1M CaCl<span class="sub">2</sub>
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|align="right"|-
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|align="right"|0.1ml
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|-
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|}
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</div>
</div>

Revision as of 12:36, 21 September 2011

Reagents

  • LB broth
reagents final conc. amount
Bacto trypton 1% 1g
NaCl 0.5% 0.5g
Yeast extracs 0.5% 0.5g
MilliQ - 100ml
Total 100ml


  • SOB broth
reagents final conc. amount
Bacto trypton 2% 20g
NaCl 0.05% 0.5g
Yeast extracs 0.5% 5g
KCl (250mM) 2.5mM 10ml
MilliQ - 1000ml
Total 1L

Before use, add 10ml Mg sol.

Mg sol.
reagents final conc. amount
MgCl2(H2O)6 1M 20.33g
MgSO4(H2O)7 1M 24.648g
MilliQ - 100ml
Total 100ml


  • 20× M9 medium
reagents final conc. amount
Na2HPO4 - 6.0g
KH2PO4 - 3.0g
NaCL - 0.5g
NH4Cl - 1.0g
MilliQ - 50ml
Total 50ml

After A.C. , add following reagents to 1L M9 medium

reagents final conc. amount
1M MgSO4 - 1.0ml
2M Glucose - 5.6ml
1% Thiamine - 1.0ml
1M CaCl2</sub> - 0.1ml

</div>

  • 50× TAE
reagents final conc. amount
Tris 2M 242g
CH3COOH 1M 57.1mL
EDTA (0.5M, pH=8.0) 0.05M 100ml
MilliQ - 1000ml
Total 1L
  • TB
reagents final conc. amount
KOH 500mM sol. 250MM 242g
PIPES 500mM sol. 10mM 2ml
CaCl2 750mM sol. 15mM 2ml
KCl 2.5M sol. 250mM 10ml
MnCl2 550mM sol. 55mM 10ml
MilliQ - 100ml
Total 100ml

Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays

Retrieved from "http://2011.igem.org/Team:UT-Tokyo/Data/Method"