Team:Groningen/project characterisation mu
From 2011.igem.org
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- | ! colspan="2" | cI autoinducing loop (BBa_K00) after 4 hours of incubation | + | ! colspan="2" | cI autoinducing loop 1 (BBa_K00) after 4 hours of incubation |
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| [[File:ci1ui.png]] | | [[File:ci1ui.png]] | ||
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| colspan=2 | Two populations are visible blabla | | colspan=2 | Two populations are visible blabla | ||
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{| class="wikitable" | {| class="wikitable" | ||
- | ! colspan="2" | cI autoinducing loop (BBa_K00) after 4 hours of incubation | + | ! colspan="2" | cI autoinducing loop 6 (BBa_K00) after 4 hours of incubation |
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- | | [[File: | + | | [[File:ci6ui.png]] |
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- | | colspan=2 | | + | | colspan=2 | Only one population is visible blabla |
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{{FooterGroningen2011}} | {{FooterGroningen2011}} |
Revision as of 22:48, 20 September 2011
Memory Units
cI Autoinducing Loops
The autoinducing loop driven by cI will function as our first memory unit. We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we measured the fluorescence in uninduced cells with a flow cytometer. To measure the activation we used a construct with the PRM promoter producing GFP.
cI autoinducing loop 6 (BBa_K00) after 4 hours of incubation | |||||||||||||||||
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Only one population is visible blabla |