Team:Paris Liliane Bettencourt/Notebook/2011/09/20/

From 2011.igem.org

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(Characterisation of T7 autoloop, RFP - GFP)
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{| border="1" class="wikitable" style="text-align: center;"
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|+3610 gfp-/3610 gfp+ in exponantial phase at 37°C
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|+T7 autoloop RFP+ Control in E-coli at 37°C
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|[[File:0914_3610_classic_w1TRANS_s5_t1.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=0min position 5 (trans image)]]
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|[[File:T7 autoloop ctrl-25.jpg|290px|thumb|center|E-Coli  at 37°C (trans image)]]
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|[[File:0914_3610_classic_w2GFP_s5_t1.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=0min position 5 (gfp image)]]
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|[[File:T7 autoloop ctrl-26.jpg|290px|thumb|center|E-Coli  at 37°C (gfp image)]]
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|[[File:T7 autoloop ctrl-27.jpg|290px|thumb|center|E-Coli  at 37°C  (rfp image)]]
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|[[File:0914_3610_classic_w1TRANS_s5_t9.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=45min position 5 (trans image)]]
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|[[File:T7 autoloop ctrl-31.jpg|290px|thumb|center|E-Coli  at 37°C (trans image)]]
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|[[File:0914_3610_classic_w2GFP_s5_t9.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=45min position 5 (gfp image)]]
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|[[File:T7 autoloop ctrl-32.jpg|290px|thumb|center|E-Coli  at 37°C (gfp image)]]
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|[[File:T7 autoloop ctrl-33.jpg|290px|thumb|center|-Coli  at 37°C (rfp image)]]
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|[[File:0914_3610_classic_w1TRANS_s5_t12.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=60min position 5 (trans image)]]
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|[[File:0914_3610_classic_w2GFP_s5_t12.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ at 37°C t=60min position 5 (gfp image)]]
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|[[File:0914_3610_classic_w1TRANS_s5_t23.png|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=180min position 5 (trans image)]]
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|[[File:0914_3610_classic_w2GFP_s5_t23.jpg|450px|thumb|center|3610 gfp- and 3610 gfp+ strains at 37°C t=180min position 5 (gfp image)]]
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TEXT
TEXT

Revision as of 20:06, 20 September 2011

Team IGEM Paris 2011


Contents

Cyrille

Control digestion of pHM3 in NcoI

CP2009.jpg



Baptiste, Hovannes & Ouriel

Characterisation of T7 autoloop, RFP - GFP

We characterized T7 autoloop by microscopy. Leak,


T7 autoloop RFP+ Control in E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)

YFP-tetR

We



TEXT


T7 autoloop RFP+ Control in E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


TEXT