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Team:Groningen/project notebook/7 September 2011
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{{HeaderGroningen2011}} {{notebookgroningen}} | {{HeaderGroningen2011}} {{notebookgroningen}} | ||
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| + | =Mon 7 September 2011= | ||
| + | '''Vessa''' | ||
| + | ==Highlight== | ||
| + | Project presentation at MI5 seminar | ||
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'''Joyce''' | '''Joyce''' | ||
<br> | <br> | ||
Revision as of 12:10, 21 September 2011
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Mon 7 September 2011
Vessa
Highlight
Project presentation at MI5 seminar
Joyce
Project is almost comming to an end, also the amount of taq polymerase is getting very low. Searching for more taq:
Borrowed dreamtaq, in the end there was enough normal taq left, but Berke needed to use the dreamtaq.
Colony PCR of PBAD-RBS-cI-LVA (2×9),PBAD-RBS-cI-LVA-DT (1×9), PBAD-LasR-LVA-DT (1×9), PBAD-RBS-GFP-DT(2×9)
Mastermix:
Taq 10× buffer: 116μl
dNTPs 10mM: 23.2μl
MgCl2: 69.6μl
Forward Biobrick primer: 23.2μl
Reverse Biobrick primer: 23.2μl
Taq DNA polymerase: 5.8μl
MQ water: 899μl
PCR conditions:
Preheated lid: 111°C
Denaturation: 94°C for 10 min.
Cycle 33×:
denaturation: 94°C for 30s.
annealing: 60°C for 30s.
Extension: 72°C for 2,5 min.
Final extension: 72°C for 10 min.
Store infinite at 4°C
Analyse on a 1% TBE agarose gel.
After analysis of the 3 gels, make two new ones and put Berke's and Christophs samples in there.
Analyse on a 1% TBE agarose gel
During the day: working on the wiki and making phone call for the church choir's general recording of the song.
Also work on the presentations for the schools.
Start making a dayplan and also work out the practicum thing for the students (how much of the solutions do we need and what
do I need to buy and make sure there is a waterbath etc.
Clones of the transformants of yesterday checked today with colony PCR look alright!:)
These colonies were grown overnight in LB medium with Cm (except 1 PBAD-RBS-GFP-DT in ampicillin vector)
PBAD-RBS-cI-LVA:1, PBAD-RBS-cI-LVA(2):1,PBAD-RBS-cI-LVA-DT: 1+3, PBAD-LasR-LVA-DT 3+5 ,PBAD-RBS-GFP-DT: 6,PBAD-RBS-GFP-DT(2): 4
Gel with Berke's and Christoph's samples finished: made a photo on the gel doc and send it to them by e-mail
At 5 there will be a presentation meeting to let the presenters practice their presentation for tomorrow's GBB symposium!
