Team:Osaka/week8

From 2011.igem.org

(Difference between revisions)
Line 17: Line 17:
#* 114(T):111(upstream)+112(downstream)+1-7A(vector)
#* 114(T):111(upstream)+112(downstream)+1-7A(vector)
# Transformation of ligation products
# Transformation of ligation products
-
# Measurment of cell survival
+
# Plating (cell survival)
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
|+Table 7
|+Table 7
!Name!!OD600!!UV(mJ/cm^2)
!Name!!OD600!!UV(mJ/cm^2)
|-
|-
-
|PprI||0.0109||0 , 0.5 , 1 , 2 , 5
+
|PprI-1||0.0109||0  
|-
|-
-
|PprA||0.0418||0 , 0.5 , 1 , 2 , 5
+
|PprI-2||0.0109||0.5
|-
|-
-
|PprM||0.0454||0 , 0.5 , 1 , 2 , 5
+
|PprI-3||0.0109||1.0
|-
|-
-
|RecA||0.0114||0 , 0.5 , 1 , 2 , 5
+
|PprI-4||0.0109||2.0
|-
|-
-
|empty vector||0.0515||0 , 0.5 , 1 , 2 , 5
+
|PprI-5||0.0109||5.0
|-
|-
-
|wild type||0.0546||0 , 0.5 , 1 , 2 , 5
+
|PprA-1||0.0418||0
 +
|-
 +
|PprA-2||0.0418||0.5
 +
|-
 +
|PprA-3||0.0418||1.0
 +
|-
 +
|PprA-4||0.0418||2.0
 +
|-
 +
|PprA-5||0.0418||5.0
 +
|-
 +
|PprM-1||0.0454||0
 +
|-
 +
|PprM-2||0.0454||0.5
 +
|-
 +
|PprM-3||0.0454||1.0
 +
|-
 +
|PprM-4||0.0454||2.0
 +
|-
 +
|PprM-5||0.0454||5.0
 +
|-
 +
|RecA-1||0.0114||0
 +
|-
 +
|RecA-2||0.0114||0.5
 +
|-
 +
|RecA-3||0.0114||1.0
 +
|-
 +
|RecA-4||0.0114||2.0
 +
|-
 +
|RecA-5||0.0114||5.0
 +
|-
 +
|empty vector-1||0.0515||0
 +
|-
 +
|empty vector-2||0.0515||0.5
 +
|-
 +
|empty vector-3||0.0515||1.0
 +
|-
 +
|empty vector-4||0.0515||2.0
 +
|-
 +
|empty vector-5||0.0515||5.0
 +
|-
 +
|wild type-1||0.0546||0
 +
|-
 +
|wild type-2||0.0546||0.5
 +
|-
 +
|wild type-3||0.0546||1.0
 +
|-
 +
|wild type-4||0.0546||2.0
 +
|-
 +
|wild type-5||0.0546||5.0
|}
|}
==September 20(Tue)==
==September 20(Tue)==
 +
# To liquid culture:113,114
# colony check (cell survival)
# colony check (cell survival)
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
Line 51: Line 100:
|-
|-
|PprI-5||5.0||
|PprI-5||5.0||
-
|-
 
|-
|-
|PprA-1||0||
|PprA-1||0||
Line 103: Line 151:
|wild type-5||5.0||
|wild type-5||5.0||
|}
|}
 +
 +
==September 21(Wed)==
==September 21(Wed)==

Revision as of 13:30, 20 September 2011


Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Plating (cell survival)
Table 7
NameOD600UV(mJ/cm^2)
PprI-10.01090
PprI-20.01090.5
PprI-30.01091.0
PprI-40.01092.0
PprI-50.01095.0
PprA-10.04180
PprA-20.04180.5
PprA-30.04181.0
PprA-40.04182.0
PprA-50.04185.0
PprM-10.04540
PprM-20.04540.5
PprM-30.04541.0
PprM-40.04542.0
PprM-50.04545.0
RecA-10.01140
RecA-20.01140.5
RecA-30.01141.0
RecA-40.01142.0
RecA-50.01145.0
empty vector-10.05150
empty vector-20.05150.5
empty vector-30.05151.0
empty vector-40.05152.0
empty vector-50.05155.0
wild type-10.05460
wild type-20.05460.5
wild type-30.05461.0
wild type-40.05462.0
wild type-50.05465.0


September 20(Tue)

  1. To liquid culture:113,114
  2. colony check (cell survival)
Table 8
NameUV(mJ/cm^2)conoly counts
PprI-10
PprI-20.5
PprI-31.0
PprI-42.0
PprI-55.0
PprA-10
PprA-20.5
PprA-31.0
PprA-42.0
PprA-55.0
PprM-10
PprM-20.5
PprM-31.0
PprM-42.0
PprM-55.0
RecA-10
RecA-20.5
RecA-31.0
RecA-42.0
RecA-55.0
Empty vector-10
Empty vector-20.5
Empty vector-31.0
Empty vector-42.0
Empty vector-55.0
wild type-10
wild type-20.5
wild type-31.0
wild type-42.0
wild type-55.0


September 21(Wed)

September 22(Thu)

September 23(Fri)

September 24(Sat)

Back to Notebook

Retrieved from "http://2011.igem.org/Team:Osaka/week8"