Team:Osaka/week8

From 2011.igem.org

(Difference between revisions)
Line 22: Line 22:
!Name!!OD600!!UV(mJ/cm^2)
!Name!!OD600!!UV(mJ/cm^2)
|-
|-
-
|PprI||0.0109||-
+
|PprI①||0.0109||0
 +
|-
 +
|PprI②||0.0109||0.5
 +
|-
 +
|PprI③||0.0109||1
 +
|-
 +
|PprI④||0.0109||2
 +
|-
 +
|PprI⑤||0.0109||5
|-
|-
|PprA||400μl||2h
|PprA||400μl||2h

Revision as of 02:19, 20 September 2011


Contents

September 18(Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:03,105,106,107,108,109,110,111,112

September 19(Mon)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
    • ok
  4. Ligation
    • 113(T):105(upstream)+110(downstream)+1-7A(vector)
    • 114(T):111(upstream)+112(downstream)+1-7A(vector)
  5. Transformation of ligation products
  6. Measurment of cell survival
Table 7
NameOD600UV(mJ/cm^2)
PprI①0.01090
PprI②0.01090.5
PprI③0.01091
PprI④0.01092
PprI⑤0.01095
PprA400μl2h
PprM400μl1h
RecA40μl2h
empty vector40μl1h
wild type40μl1h


September 20(Tue)

September 21(Wed)

September 22(Thu)

September 23(Fri)

September 24(Sat)

Back to Notebook

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