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Team:Cornell/Week 16
From 2011.igem.org
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Afternoon-2 lab work done by: Charlie Chung | Afternoon-2 lab work done by: Charlie Chung | ||
:'''Background''' | :'''Background''' | ||
| - | ::*The PCR site-directed mutagenesis done earlier today should ideally enhance protein expression of VioA, VioB, VioE, and RFP | + | ::*The PCR site-directed mutagenesis done earlier today should ideally enhance protein expression of VioA, VioB, VioE, and RFP by deleting excessive nucleotides between the ribosome binding site and the ATG start codon in the original DNA sequences |
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:'''Work Accomplished''' | :'''Work Accomplished''' | ||
::*Plated the transformed products of VioA, VioB, VioE, and RFP on agar plates treated with ampicillin or carbenicillin (two are on amp; other two are on carb) | ::*Plated the transformed products of VioA, VioB, VioE, and RFP on agar plates treated with ampicillin or carbenicillin (two are on amp; other two are on carb) | ||
Revision as of 23:42, 19 September 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
September 18th - September 24th
Sunday, September 18
Monday, September 19
Morning lab work done by: Jim Mathew
- ...
Afternoon-1 lab work done by: Youjin Cho
- ...
Afternoon-2 lab work done by: Charlie Chung
- Background
- The PCR site-directed mutagenesis done earlier today should ideally enhance protein expression of VioA, VioB, VioE, and RFP by deleting excessive nucleotides between the ribosome binding site and the ATG start codon in the original DNA sequences
- Work Accomplished
- Plated the transformed products of VioA, VioB, VioE, and RFP on agar plates treated with ampicillin or carbenicillin (two are on amp; other two are on carb)
- Incubating at 37°C in Olin 303
