Team:Paris Bettencourt/Experiments

From 2011.igem.org

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<h1>Demonstrative experiments</h1>
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<h1>Experiments</h1>
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<p>In the Dubey and Ben-Yehuda paper <a href="http://bms.ucsf.edu/sites/ucsf-bms.ixm.ca/files/marjordan_06022011.pdf">[1]</a> a set of simple experiments are made to prove the existence of the nanotubes. We tryied to reproduce some of them in order to demonstrate again the existence of these entities as a medium of communication between bacteria, and to be sure we are in the good experimental conditions to produce them and that we can go on with our designs.</p>
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<p>You can find here all the experiments we did in the lab! The data is organized by design and you will be able to find both the characterization steps and our diffusion experiments.</p>
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<p>We tryied to reproduce the two keystone experiments of the paper: the GFP diffusion, and the antibiotic resistance exchange, with less sucess for the latter.</p>
 
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   <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/GFP_diff"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/8/80/GFP-diff-button.png"></a>
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   <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Preliminaries"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/8/80/GFP-diff-button.png"></a>
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   <td><b>The <a href="https://2011.igem.org/Team:Paris_Bettencourt/GFP_diff">GFP diffusion experiment</a></b> is the simplest experiment possible. One Bacillus Subtlis strain that produce GFP is mixed with a wild type strain. If some nanotubes are formed, the GFP will diffuse through the tubes and color the non fluorescent strain. We invite you to <a href="https://2011.igem.org/Team:Paris_Bettencourt/GFP_diff">visit corresponding the page</a> to learn more about what we did and the results we had.
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   <td><b>The <a href="https://2011.igem.org/Team:Paris_Bettencourt/Preliminaries">Preliminaries experiments<a></b>: go back and see how we re-did the experiment of the original nanotubes paper.
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Revision as of 15:52, 19 September 2011

Team IGEM Paris 2011