User:Allancrossman

Allan Crossman

Allan Crossman, member of Edinburgh 2011.

Personal TODO list

• https://igem.org/2011_Judging_Form?id=523
• Finish RFC 81 and mention it on Wiki.
• We are need poster.

Also:

• "Likewise should be easy to get nice pics of the Plac-cenA (Congo Red), Plac-cex (MUC) and Plac-rcsA (mucoid) colonies."

Less important:

• Fix Lukasz's animation page?
• The sitemap needs to be updated or eliminated.
• Suppress hints box if no hints present.
• Hints box needs to go up as high as it can.

Notes to self

• [http://mic.sgmjournals.org/content/142/7/1659.full.pdf+html bglX info]
• We must have a "data page" that explains the system and links to actual data which must be on the Registry.
• We must have (somewhere) a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.
• User:Allancrossman/Gibson and User:Allancrossman/Gibson2
• inaZ is [http://www.ncbi.nlm.nih.gov/nuccore/71553748?from=1850733&to=1855854 here], broken by an IS.
• [http://www.lifesci.dundee.ac.uk/groups/tracy_palmer/docs/signals.htm Substrates of TAT pathway in E. coli]
• RBS strengths from Warsaw 2010
• [http://www.ncbi.nlm.nih.gov/nuccore/AF130864.1 pG8SAET]
• Contributions: Yassen
• Contributions: Lukasz

Large display of small GIF

Non-synergistic versus synergistic systems. Dark regions are places where the cellulose has been degraded down to free glucose molecules.

Video game concept

You control a humble ribosome. You are powered by sugar. Outside the cell is cellobiose floating around. You can express B-glucosidase on the cell exterior to get more sugar. You can express other stuff. There's cellulose floating around too. You can express cellulases. There's phage. You can express proteases or somesuch?

Lignin degradation

• Requires glucose oxidase to supply H2O2, a cofactor for other enzymes...
  • Lignin peroxidase
  • Manganaese peroxidase
  • Laccase?

Some of these use flavin cofactors but it seems proteins using these can be successfully displayed using INP (Van Bloois et al, 2009).

Gold medal criteria

• Our wiki watch page is clearly a type of collaboration.
• Cooperation with Trieste re: C. fimi BGlu sequence.
• Cooperation with Trieste re: C. fimi BGlu assay.
• Registry update for K415151.
• Registry update for K392008.
• Registry update for K265008.
• Registry update for K118022.

References

• Van Bloois E, Winter RT, Janssen DB, Fraaije MW (2009) [http://www.springerlink.com/content/d471504054865565/fulltext.pdf Export of functional Streptomyces coelicolor alditol oxidase to the periplasm or cell surface of Escherichia coli and its application in whole-cell biocatalysis]. Applied Microbiology and Biotechnology 83: 679-687 (doi: 10.1007/s00253-009-1904-0).