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Team:Imperial College London/Protocols Switch
From 2011.igem.org
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<h1>Gene Guard</h1> | <h1>Gene Guard</h1> | ||
| - | + | <h2>Genome integration</h2> | |
| + | <p>-Grow a culture of CRIM helper plasmid containing cells and make them competent.</p> | ||
| + | |||
| + | <p>-Transform competent cells with CRIM plasmid.</p> | ||
| + | |||
| + | <p>-After transformation, suspend cells in SOC without ampicillin.</p> | ||
| + | |||
| + | <p>-Incubate at 37°C for one hour.</p> | ||
| + | |||
| + | <p>-Incubate at 42°C for 30 minutes.</p> | ||
| + | |||
| + | <p>-Plate cells on agar plates containing kanamycin.</p> | ||
<h2>Soil survivability</h2> | <h2>Soil survivability</h2> | ||
Revision as of 15:45, 19 September 2011
Protocols
This page lists all the protocols used in our project. We have classified them into five main categories as follow.
Gene Guard
Genome integration
-Grow a culture of CRIM helper plasmid containing cells and make them competent.
-Transform competent cells with CRIM plasmid.
-After transformation, suspend cells in SOC without ampicillin.
-Incubate at 37°C for one hour.
-Incubate at 42°C for 30 minutes.
-Plate cells on agar plates containing kanamycin.
