Team:UQ-Australia/Data

(Difference between revisions)

Revision as of 11:58, 4 October 2011

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We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows:

1: 1kb+ ladder

2: Negative control (H2O)

3-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

8: Negative control (H2O)

9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

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+We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows:
+: 1kb+ ladder
+: Negative control (H2O)
+-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.
+: Negative control (H2O)
+-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.
+[[File:IMG_0190.JPG | 396x202px]]