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Team:Sevilla/Week5
From 2011.igem.org
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<p>Purification of the digestions with ethanol before ligations has proven to be important, but we still don't get the protocol right. Give us some time to optimize it!</p> | <p>Purification of the digestions with ethanol before ligations has proven to be important, but we still don't get the protocol right. Give us some time to optimize it!</p> | ||
| - | <p>However, the second PCR has been a complete success. We have to purify the DNA from the gel - a new protocol should be exciting, but it's becoming more like a nightmare!</p> | + | <p>However some good news, the second PCR has been a complete success. We have to purify the DNA from the gel - a new protocol should be exciting, but it's becoming more like a nightmare!</p> |
<input type="button" value="Show Monday protocols" id="mondayBP"> | <input type="button" value="Show Monday protocols" id="mondayBP"> | ||
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The purifications from gel didn't go as expected, and to make matters worse the whole department of Genetics is in a complete chaos; there's no floor, all the important machines are hidden, and we don't have access to most of the rooms. It seems it won't get any better during the week... | The purifications from gel didn't go as expected, and to make matters worse the whole department of Genetics is in a complete chaos; there's no floor, all the important machines are hidden, and we don't have access to most of the rooms. It seems it won't get any better during the week... | ||
</p> | </p> | ||
| + | |||
<input type="button" value="Show Tuesday protocols" id="tuesdayBP"> | <input type="button" value="Show Tuesday protocols" id="tuesdayBP"> | ||
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<p> | <p> | ||
| - | A freshly cemented corridor separates us from the door behind which our PCR reactions are waiting for us to rescue them. | + | A freshly cemented corridor separates us from the door behind which our PCR reactions are waiting for us to rescue them. As well as this, getting to the NanoDrop or the Transiluminator rooms has become an adventure due to the piles of rubble and the hanging wires we have to dodge. This department has become a modern jungle, so we try not to come over here, and stay in our downstairs lab working on more digestiones and electrophoresis. |
</p> | </p> | ||
| + | |||
<input type="button" value="Show Wednesday protocols" id="wednesdayBP"> | <input type="button" value="Show Wednesday protocols" id="wednesdayBP"> | ||
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<p> | <p> | ||
| - | The department of Genetics is hardly accessible today, but things aren't better here in the lab: our fridge doesn't work properly and now we have all our chemicals and DNA samples surrounded by blocks of ice. | + | The department of Genetics is hardly accessible at all today, but things aren't any better here in the lab: our fridge doesn't work properly and now we have all our chemicals and DNA samples surrounded by blocks of ice. |
</p> | </p> | ||
| - | <p> How to fix a freezer, lesson one: gather some mops, ice trays to keep things cool and something to crush the blocks until they come unstuck. The rest is easy: open the freezer | + | <p> How to fix a freezer, lesson one: gather some mops, ice trays to keep things cool and something to crush the blocks until they come unstuck. The rest is easy: open the freezer door, put your chemicals in the trays, and then charge, charge, charge at the blocks and let the ice melt. Then clean up all the mess and voila, you'll have plenty of space for all your digestions, biobricks, etc. |
</p> | </p> | ||
<input type="button" value="Show Thursday protocols" id="thursdayBP"> | <input type="button" value="Show Thursday protocols" id="thursdayBP"> | ||
Revision as of 06:01, 19 September 2011
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Monday 8 August
Purification of the digestions with ethanol before ligations has proven to be important, but we still don't get the protocol right. Give us some time to optimize it!
However some good news, the second PCR has been a complete success. We have to purify the DNA from the gel - a new protocol should be exciting, but it's becoming more like a nightmare!
Monday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Tuesday 9 August
The purifications from gel didn't go as expected, and to make matters worse the whole department of Genetics is in a complete chaos; there's no floor, all the important machines are hidden, and we don't have access to most of the rooms. It seems it won't get any better during the week...
Tuesday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Wednesday 10 August
A freshly cemented corridor separates us from the door behind which our PCR reactions are waiting for us to rescue them. As well as this, getting to the NanoDrop or the Transiluminator rooms has become an adventure due to the piles of rubble and the hanging wires we have to dodge. This department has become a modern jungle, so we try not to come over here, and stay in our downstairs lab working on more digestiones and electrophoresis.
Wednesday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Thursday 11 August
The department of Genetics is hardly accessible at all today, but things aren't any better here in the lab: our fridge doesn't work properly and now we have all our chemicals and DNA samples surrounded by blocks of ice.
How to fix a freezer, lesson one: gather some mops, ice trays to keep things cool and something to crush the blocks until they come unstuck. The rest is easy: open the freezer door, put your chemicals in the trays, and then charge, charge, charge at the blocks and let the ice melt. Then clean up all the mess and voila, you'll have plenty of space for all your digestions, biobricks, etc.
Thursday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Friday 12 August
We finally got some ligations right, but they're too few and we're not sure whether they're OK. We'll have to study them carefully...
Friday Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
Weekend
wwwwwwwwwwwwwwwwRhoncus nisi adipiscing, rhoncus. Porttitor urna rhoncus? A placerat scelerisque mattis lectus tristique magna pellentesque ac ac, et? Adipiscing penatibus porta. Turpis ultrices lorem vel vel massa elementum! Lorem vel lectus et integer porttitor magna aenean, quis magnis turpis aliquam? Sed enim? Placerat velit sit turpis penatibus enim. Enim cursus hac massa eu a cum etiam? Amet pid porta non tincidunt cum pellentesque eu a pid nisi ultrices? Lectus augue dictumst purus, velit scelerisque vut. A porttitor turpis, natoque aliquam elementum. Tristique, mauris turpis? Arcu tristique sed massa est nunc purus pellentesque, risus nec rhoncus, scelerisque ac urna porttitor, sit.
Weekend Protocols
In. Mid vel vel elementum, placerat tincidunt, vut lacus purus nunc massa a habitasse? Ac pid elementum aliquam, natoque! Porttitor facilisis amet lacus, ridiculus nascetur! Sociis vel aenean natoque penatibus aliquam in, augue mauris, dictumst, nunc mid platea a! Massa? Vut dis purus. Odio magna nec. Lorem ut turpis adipiscing scelerisque dis, scelerisque. Sed quis, porttitor et pulvinar in. Massa sit, porta urna nunc auctor non elementum! Augue dolor cum sociis, dignissim elementum. Integer. Penatibus. Phasellus et porta nec! Hac in nec porttitor ridiculus rhoncus. Hac natoque mid est nunc platea nisi sed habitasse! Amet nec placerat ultricies egestas. Vel cum diam, lorem integer et montes, nec placerat, turpis lacus nec placerat, rhoncus rhoncus, a porta augue ac! Et aliquet. Porttitor diam egestas, non vut etiam montes, aliquet? Pellentesque. Dolor nascetur amet. Adipiscing sed pulvinar cursus! Urna in, in turpis duis in sit placerat, vut non aliquam? Mattis habitasse augue mus. Pulvinar auctor natoque est lacus porta sagittis! A in massa lundium? In non, lundium nisi magna proin elementum sed porttitor sed amet risus a aliquet mid a? Proin turpis, ac magna, dictumst lorem tincidunt penatibus sociis rhoncus? Duis cursus sit diam amet nisi mattis egestas habitasse diam.
