Team:Paris Bettencourt/Modeling/Parameters
From 2011.igem.org
(Difference between revisions)
Line 26: | Line 26: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><img src='https://static.igem.org/mediawiki/2011/6/63/Beta_const.png' style='height: | + | <td><img src='https://static.igem.org/mediawiki/2011/6/63/Beta_const.png' style='height:28px;' /></td> |
<td>Maximal production rate of pVeg promoter (constitutive)</td> | <td>Maximal production rate of pVeg promoter (constitutive)</td> | ||
<td>0.02</td> | <td>0.02</td> | ||
Line 33: | Line 33: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><img src='https://static.igem.org/mediawiki/2011/d/d9/BetapLac.png' style='height: | + | <td><img src='https://static.igem.org/mediawiki/2011/d/d9/BetapLac.png' style='height:28px;' /></td> |
<td>Maximal production rate of pHyperSpank promoter</td> | <td>Maximal production rate of pHyperSpank promoter</td> | ||
<td>0.02</td> | <td>0.02</td> | ||
Line 40: | Line 40: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td><img src='https://static.igem.org/mediawiki/2011/5/5d/BetapT7.png' style='height: | + | <td><img src='https://static.igem.org/mediawiki/2011/5/5d/BetapT7.png' style='height:28px;' /></td> |
<td>Maximal production rate of pT7 promoter</td> | <td>Maximal production rate of pT7 promoter</td> | ||
<td>0.02</td> | <td>0.02</td> |
Revision as of 12:48, 16 September 2011
Parameters
Parameter | Description | Value | Unit | Reference |
---|---|---|---|---|
Concentration of X | NA | molecules per cell |
Notation convention | |
Delay due to protein X production and maturation | NA | s | Notation convention | |
Maximal production rate of pVeg promoter (constitutive) | 0.02 | molecules.s-1 or pops |
Estimated, see the justification | |
Maximal production rate of pHyperSpank promoter | 0.02 | molecules.s-1 or pops |
Estimated, see the justification | |
Maximal production rate of pT7 promoter | 0.02 | molecules.s-1 or pops |
Estimated, see the justification | |
Dissociation constant for IPTG to LacI | 1200 | molecules per cell |
Aberdeen 2009 wiki | |
Dissociation constant for LacI to LacO (pLac) | 700 | molecules per cell |
Aberdeen 2009 wiki | |
Dissociation constant for T7 RNA polymerase to pT7 | 10 | molecules per cell |
We used the classic assumption 1nM=1 molecule per cell and [1] | |
Translation rate of proteins | 0.9 | s-1 | Estimated, see the justification | |
Dilution rate in exponential phase | 2.88x10-4 | s-1 | Calculated with a 40 min generation time. See explanation | |
Degradation rate of mRNA | 2.88x10-3 | s-1 | Uri Alon (To Be Confirmed) | |
Degradation rate of GFP | 10-4 | s-1 | BioNumbers | |
Degradation rate of RFP | 10-4 | s-1 | Estimated equal to GFP degradation rate | |
Delay due tT7 RNA polymerase production and maturation | 300 | s | [2] | |
Delay due GFP production and maturation | 360 | s | BioNumbers | |
Delay due RFP production and maturation | 360 | s | Estimated equal to GFP delay (similar molecules) | |
Delay due to mRNA production | 30 | s | BioNumbers with an approximation: all our contructs are around 1-2kb |
References