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Team:Imperial College London/Sequence
From 2011.igem.org
| Line 3: | Line 3: | ||
<body> | <body> | ||
<h1>Sequencing Results</h1> | <h1>Sequencing Results</h1> | ||
| - | < | + | <h2>Vector 7 forward sequencing result</h2> |
<p> BBa_K316001 (7)_premix -- 19..973 of sequence </p> | <p> BBa_K316001 (7)_premix -- 19..973 of sequence </p> | ||
| Line 24: | Line 24: | ||
CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA</p> | CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA</p> | ||
| - | < | + | <h2>Vector 8 forward sequencing result</h2> |
<p>BBa_K316005 (8)_premix -- 17..635 of sequence</p> | <p>BBa_K316005 (8)_premix -- 17..635 of sequence</p> | ||
| Line 39: | Line 39: | ||
CTGGAAAGTGGGGTTTGAA</p> | CTGGAAAGTGGGGTTTGAA</p> | ||
| - | < | + | <h2>Vector 6 forward sequencing result</h2> |
<p>BBa_K398500 (6)_premix -- 17..960 of sequence</p> | <p>BBa_K398500 (6)_premix -- 17..960 of sequence</p> | ||
| Line 59: | Line 59: | ||
ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT</p> | ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT</p> | ||
| - | < | + | <h2>?</h2> |
<p>PSVi34-1_premix -- 19..951 of sequence</p> | <p>PSVi34-1_premix -- 19..951 of sequence</p> | ||
| Line 79: | Line 79: | ||
AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG</p> | AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG</p> | ||
| - | < | + | <h2>?</h2> |
<p>PSVi34-2_premix -- 21..949 of sequence</p> | <p>PSVi34-2_premix -- 21..949 of sequence</p> | ||
| Line 99: | Line 99: | ||
GCTAAGATCCGCTCTAACCGAAAAGGAAG</p> | GCTAAGATCCGCTCTAACCGAAAAGGAAG</p> | ||
| - | < | + | <h2>Sequence results for J23113 ligation attempt into RFP. Failed.</h2> |
<p> 52.E_premix -- 14..957 of sequence</p> | <p> 52.E_premix -- 14..957 of sequence</p> | ||
| Line 118: | Line 118: | ||
TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG | TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG | ||
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT</p> | CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT</p> | ||
| + | |||
| + | <h2>Sequence results for possible auxin producing colony</h2> | ||
<p>53_AUX1 -- 13..914 of sequence</p> | <p>53_AUX1 -- 13..914 of sequence</p> | ||
| Line 168: | Line 170: | ||
GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA | GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA | ||
AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA</p> | AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA</p> | ||
| - | |||
| - | |||
| - | |||
<p>53_premix -- 17..119 of sequence</p> | <p>53_premix -- 17..119 of sequence</p> | ||
| Line 193: | Line 192: | ||
| - | < | + | <h2>Sequence results for possible auxin producing colony</h2> |
<p>55_AUX1 -- 18..841 of sequence</p> | <p>55_AUX1 -- 18..841 of sequence</p> | ||
| Line 278: | Line 277: | ||
ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT</p> | ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT</p> | ||
| - | < | + | <h2>Sequence results for our Auxin Xpress module.</h2> |
<p>56_AUX1 -- 21..841 of sequence</p> | <p>56_AUX1 -- 21..841 of sequence</p> | ||
| Line 295: | Line 294: | ||
CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA | CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA | ||
CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA</p> | CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA</p> | ||
| - | |||
| - | |||
<p>56_AUX2 -- 16..912 of sequence</p> | <p>56_AUX2 -- 16..912 of sequence</p> | ||
| Line 367: | Line 364: | ||
TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG | TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG | ||
CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC</p> | CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC</p> | ||
| + | |||
| + | <h2>Sequence results to determine SpeI site problem in sfGFP</h2> | ||
<p>17R_premix -- 35..933 of sequence</p> | <p>17R_premix -- 35..933 of sequence</p> | ||
| Line 385: | Line 384: | ||
GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA</p> | GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA</p> | ||
| - | < | + | <h2>Sequence results for possible PA2652 producing colony</h2> |
<p>57.20F_premix -- 14..986 of sequence</p> | <p>57.20F_premix -- 14..986 of sequence</p> | ||
| Line 425: | Line 424: | ||
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG</p> | TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG</p> | ||
| - | < | + | <h2>Sequence results for possible PA2652 producing colony</h2> |
<p>57.3F_premix -- 15..985 of sequence</p> | <p>57.3F_premix -- 15..985 of sequence</p> | ||
| Line 465: | Line 464: | ||
TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT</p> | TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT</p> | ||
| - | < | + | <h2>Sequence results for possible PA2652 producing colony</h2> |
<p>57.7F_premix -- 14..987 of sequence</p> | <p>57.7F_premix -- 14..987 of sequence</p> | ||
| Line 505: | Line 504: | ||
CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC</p> | CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC</p> | ||
| - | < | + | <h2>Sequence results for our PA2652 producing construct. Correct.</h2> |
<p>57.8F_premix -- 15..979 of sequence</p> | <p>57.8F_premix -- 15..979 of sequence</p> | ||
| Line 545: | Line 544: | ||
TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG</p> | TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG</p> | ||
| + | <h2>Dendra2 forward sequencing results</h2> | ||
| + | <p> Dendra_forward_premix -- 15..179 of sequence</p> | ||
| + | <p>TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA | ||
| + | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATTGACA | ||
| + | TCCCTATCAGTGATAGAGATACTGAGCACTACTAGATGAACATCA</p> | ||
| + | <h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2> | ||
| + | <p> 21+60a_frwrd_premix -- 15..921 of sequence</p> | ||
| + | <p>ATAATAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA | ||
| + | AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGA | ||
| + | TTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATC | ||
| + | AAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAA | ||
| + | ATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTT | ||
| + | ACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGT | ||
| + | TCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCG | ||
| + | GAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACC | ||
| + | CAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAAC | ||
| + | TTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAA | ||
| + | CGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAA | ||
| + | GACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAG | ||
| + | CTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC | ||
| + | ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGC | ||
| + | TGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCG | ||
| + | CTGCAGG</p> | ||
| + | |||
| + | <h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2> | ||
| + | |||
| + | <p> 21+60b_frwrd_premix -- 18..928 of sequence</p> | ||
| + | <p>TATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG | ||
| + | ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA | ||
| + | TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA | ||
| + | GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC | ||
| + | GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC | ||
| + | AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC | ||
| + | AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA | ||
| + | GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG | ||
| + | GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC | ||
| + | CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT | ||
| + | ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC | ||
| + | GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG | ||
| + | CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC | ||
| + | ATCGTTGAACAGTACGAACGTGCTGAAAGGTCGTCACTCCACCGGTGCTTAATAACGCTG | ||
| + | ATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCT | ||
| + | GCAGGCTTCCT</p> | ||
| + | |||
| + | <h2>Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange</h2> | ||
| + | |||
| + | <p> 21+60c_frwrd_premix -- 18..926 of sequence</p> | ||
| + | <p>AAGATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG | ||
| + | ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA | ||
| + | TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA | ||
| + | GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC | ||
| + | GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC | ||
| + | AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC | ||
| + | AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA | ||
| + | GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG | ||
| + | GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC | ||
| + | CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT | ||
| + | ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC | ||
| + | GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG | ||
| + | CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC | ||
| + | ATCGTTGAACAGTACGAACGTGCTGAAGTCGTCACTCCACCGGTGCTTAATAACGCTGAT | ||
| + | AGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCTGC | ||
| + | AGGCTTCCT</p> | ||
| + | |||
| + | <h2>Results of corrected SpeI site of sfGFP</h2> | ||
| + | |||
| + | <p> sfGFP_reverse_premix -- 20..288 of sequence</p> | ||
| + | <p>ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT | ||
| + | TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG | ||
| + | CTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCAC | ||
| + | AAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTG | ||
| + | GGTGCTCAGATAGTGATTATGCCGGAGCC</p> | ||
| - | < | + | <h2>Sequence of Anti-holin colony 2</h2> |
<p> 70.2 F_premix -- 25..943 of sequence</p> | <p> 70.2 F_premix -- 25..943 of sequence</p> | ||
<p>AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG | <p>AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG | ||
| Line 587: | Line 658: | ||
GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT</p> | GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT</p> | ||
| - | < | + | <h2>Sequence of Anti-holin colony 2</h2> |
<p> 70.9 F_premix -- 18..1008 of sequence</p> | <p> 70.9 F_premix -- 18..1008 of sequence</p> | ||
Revision as of 09:12, 16 September 2011
Sequencing Results
Vector 7 forward sequencing result
BBa_K316001 (7)_premix -- 19..973 of sequence
TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG ACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTGACAAAAATGGGCTCGTGTT GTACAATAAATGTTACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAAGGTGTCA CCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCG CTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAG GCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTC CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCT CATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAG TCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGGTAACAGGATTAG CAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA CACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGA
Vector 8 forward sequencing result
BBa_K316005 (8)_premix -- 17..635 of sequence
TAGAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA CAATAAATGTTACTAGAGCTCATGAACTATGAAGAGGTGACGTCATGAACAAAGGTGTAA TGCGACCGGGCCATGTGCAGCTGCGTGTACTGGACATGAGCAAGGCCCTGGAACACTACG TCGAGTTGCTGGGCCTGATCGAGATGGACCGTGACGACCAGGGCCGTGTCTATCTGAAGG CTTGGACCGAAGTGGATAAGTTTTCCCTGGTGCTACGCGAGGCTGACGAGCCGGGCATGG ATTTTATGGGTTTCAAGGTTGTGGATGAGGATGCTCTCCGGCAACTGGAGCGGGATCTGA TGGCATATGGCTGTGCCGTTGAGCAGCTACCCGCAGGTGAACTGAACAGTTGTGGCCGGC GCGTGCGCTTCCAGGCCCCCTCCGGGCATCACTTCGAGTTGTATGCAGACAAGGAATATA CTGGAAAGTGGGGTTTGAA
Vector 6 forward sequencing result
BBa_K398500 (6)_premix -- 17..960 of sequence
TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCTTCTAGAGAAAGAGGGGACAAACTAGATGCGTAAAGGAGAAGAACTTTTC ACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCT GTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGC ACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTT CAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATG CCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACA CGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATT GATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACAC AATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGA CACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATT GGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCG AAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGG ATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAA ACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT
?
PSVi34-1_premix -- 19..951 of sequence
CATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCT CAGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAA TTACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTAT TCACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTT CTGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTT GTACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTG TTCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCA TGCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGA CCAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTA TTGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTC ACAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTA GACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAA TTGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTAT CCAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTG GTATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCT AGCTAAGATCCGCTCTAACCGAAAAGGAAGGAG
?
PSVi34-2_premix -- 21..949 of sequence
ATGAATGTCTTTGTACGAAACTCATTACCCGCATTAATATTTTTTTTCTTTTTAAAGCTC AGTTGACCCTTTCTCATTCCCTTCTTAAAACAACTGTGTGATCCTTGAGAAAAGATAAAT TACATACACAACATAAACCCAACTACTAGTTATTAAAATGTCTAAAGGTGAAGAATTATT CACTGGTGTTGTCCCAATTTTGGTTGAATTAGATGGTGATGTTAATGGTCACAAATTTTC TGTCTCCGGTGAAGGTGAAGGTGATGCTACTTACGGTAAATTGACCTTAAAATTTATTTG TACTACTGGTAAATTGCCAGTTCCATGGCCAACCTTAGTCACTACTTTCGGTTATGGTGT TCAATGTTTTGCGAGATACCCAGATCATATGAAACAACATGACTTTTTCAAGTCTGCCAT GCCAGAAGGTTATGTTCAAGAAAGAACTATTTTTTTCAAAGATGACGGTAACTACAAGAC CAGAGCTGAAGTCAAGTTTGAAGGTGATACCTTAGTTAATAGAATCGAATTAAAAGGTAT TGATTTTAAAGAAGATGGTAACATTTTAGGTCACAAATTGGAATACAACTATAACTCTCA CAATGTTTACATCATGGCTGACAAACAAAAGAATGGTATCAAAGTTAACTTCAAAATTAG ACACAACATTGAAGATGGTTCTGTTCAATTAGCTGACCATTATCAACAAAATACTCCAAT TGGTGATGGTCCAGTCTTGTTACCAGACAACCATTACTTATCCACTCAATCTGCCTTATC CAAAGATCCAAACGAAAAGAGAGACCACATGGTCTTGTTAGAATTTGTTACTGCTGCTGG TATTACCCATGGTATGGATGAATTGTACAAATAACTCGAGTAAGCTTGGTACCGCGGCTA GCTAAGATCCGCTCTAACCGAAAAGGAAG
Sequence results for J23113 ligation attempt into RFP. Failed.
52.E_premix -- 14..957 of sequence
ATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCC TCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAAC GGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACC GCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCG CAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTG AAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGT GTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAA CTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGG GAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATG CGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCT AAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCC CACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACC GGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAA TACTAGTAGCGGCCGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTT
Sequence results for possible auxin producing colony
53_AUX1 -- 13..914 of sequence
GCATCTGATTTTCCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATG AGCACCAGCATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACG TGCCTGAACGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTG CCCCGCGCCCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAG ATAATTGGGGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGC TTACAACGCAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGG ATCGCACGCGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGA AGGACAAAGATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAA CGCGCGGCACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTT ACTCATGGATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAAT TAGCTTTAGATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTG GTGACCCGATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCG CGTTACCGGGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGACAT GCCGTCAGAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGGACACCGGCATG GGTTGAGGGAGCAGTACAAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGG AG
53_AUX2 -- 16..895 of sequence
GGACAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAAC CCACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGG GGACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATG ATTACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTG CGCGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAA GGCGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGG GGGGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGT ACCCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAA GCATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACG AGCATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGA GGTTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGG ACGGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGC CCTACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTG TGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGA AAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG
53_AUX3 -- 15..793 of sequence
GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAA
53_premix -- 17..119 of sequence
GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTG
53_premix -- 15..843 of sequence
ATATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAG GATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGACA ACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTGTA CAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATATAT GTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTTGG GTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCAGG CTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCCTC CAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCGGA ACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAACT GGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTGAT TGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTCCA AGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATTCA ACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGGAACTGTGGACCAAACTCGTGC CTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGC
Sequence results for possible auxin producing colony
55_AUX1 -- 18..841 of sequence
ATTTTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAG CATCTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAA CGCTGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGC CCAGGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGG GGCGATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACG CAGATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACG CGTTACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAA GATCCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGC ACATACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGG ATGAGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTA GATGCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCG ATTACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCG GGCCATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCA GAACAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA
55_AUX2 -- 16..893 of sequence
CAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCCA CGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGGA CTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGATT ACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCGC GAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGGC GACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGGG GTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTACC CGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGCA TTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAGC ATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGGT TCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGACG GACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCCT ACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAAGGATTGTGT CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCG
55_AUX3 -- 13..851 of sequence
GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGC
55_premix -- 16..869 of sequence
ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAAA CGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGACC GACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGGC CTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCGT CGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCAG CAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCAG ATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGAT GCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACAG CGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAAC AAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTTC GCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGATT AAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAGC TGTGCGCGTCAGAG
55_premix -- 14..888 of sequence
CTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTG ACAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTT GTACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATA TATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACT TGGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGC AGGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGC CTCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGC GGAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAA ACTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGT GATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTT CCAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATAT TCAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGT GCCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAA ATTAAGCTTTTACCATCGTGAGGTTTCGGACAGTT
Sequence results for our Auxin Xpress module.
56_AUX1 -- 21..841 of sequence
TTCAATAGTATGTTAGAAATCTTTCGTGTTGTGATGACGAATTGTGATGAGCACCAGCAT CTGATTGTTGGTGGCGTACAGCAAGTGCCTGTAGGCCTGTGGTCGCACGTGCCTGAACGC TGCGCACATTGGCCAAAGGGTACGTCTCTTTCCTCACTTCATCGGGGTGCCCCGCGCCCA GGTGTCAAACGTATTGCACGTGCTGAAGATGGTTCATTTACGGTTACAGATAATTGGGGC GATACACGTCAATACGCTGCCGTTTTGACAACCTGTCAATCGTGGCTGCTTACAACGCAG ATTGAATGTGAGGAATCTTTGTTTTCACAGAAGATGTGGATGGCGCTGGATCGCACGCGT TACATGCAGTCTTCTAAAACTTTTGTGATGGTTGATCGGCCGTTTTGGAAGGACAAAGAT CCGGAAACGGGTCGCGATCTTATGTCCATGACCCTCACCGATCGCTTAACGCGCGGCACA TACTTATTTGATAATGGCGACGACAAGCCAGGCGTTATTTGCCTGAGTTACTCATGGATG AGTGACGCTTTAAAAATGTTACCGCAACCGATCGAGAAACGCGTCAAATTAGCTTTAGAT GCCCTTAAAAAGATTTACCCGAAAGTGGATATCGCCGCACGTATTATTGGTGACCCGATT ACTGTGAGTTGGGAAGCGGATCCGCATTTCCTGGGGGCATTTAAAGGCGCGTTACCGGGC CATTACCGTTACAACCAGCGCATGTACGCGCATTTTATGCAGCAGGACATGCCGTCAGAA CAGCGTGGCATGTTTATTGCCGGTGATGATGTATCTTGACA
56_AUX2 -- 16..912 of sequence
GCAGTACAACGTCATTGAACGCGGTGTGGGGCATCATGAACCATTTCGGAGGGAAAACCC ACGCAGAGAATCCGGGTCCTGGCGACGTTTTCCATGAGATTGGACCGATCGCGCTGGGGG ACTAATAATCGCTGGGACGCCCGCCCCTAGAAAAGGAGGACTAAAAATGCGCGAAATGAT TACGCTTGAATCTCTGTGCCGCGCATTGGCAGACGAACAGATTGCCGCAGAAGAATTGCG CGAGCGCGCGCTGGATACAGAGGCTCGCTTAACACTGTTAAATTGCTTCATTCGCGAAGG CGACGCCGTAAGCCAATTCGGTGAAGCGGATCAGGCGCGTAAAGGAACTTCACTTTGGGG GGTGCCTGTGAGTTTTAAGGATAACATCTGTGTTCGCGGGCTGCCACTGACGGCGGGTAC CCGCGGCATGAGCGGCTTTATTGCCGATCAGGATGCAGCAATCGTGAGCCAGTTAAAAGC ATTGGGAGCTGTGGTCGCGGGCAAAAATAACATGCATGAGCTGTCATTTGGCGTCACGAG CATCAATCCACATTGGGGCGCGGTTGGAAACCCGGTGGCTCCAGGATATTGCGCCGGAGG TTCTAGCGGAGGTTCCGCTGCGGCGGTCGCATCTGGAATTGTCCCGCTCAGCGTCGGGAC GGACACAGGCGGCTCAATCCGTATTCCGGCGGCATTTTGCGGCATTACGGGTTTTCGCCC TACTACAGGTCGCTTGAGTACGGCGGGGATCATTCCGGTCAGCCACACGAAGGATTGTGT CGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGCTGTCAGGAAA ACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGCCGGTGAG
56_AUX3 -- 15..922 of sequence
GATGATGTGTCGGCCTCCTGACGCGCACAGCTGGCGACGCTGAATTTGTGTATGGGCTGC TGTCAGGAAAACAGCAGAGTTTCCCTCTTAATCGCACGGGACCTTGCCGCATCGGCCTGC CGGTGAGCATGTGGTCTGACTTAGATGGCGAAGTAGAACGTGCCTGTATCAATGCGCTGA GCCTCTTACGCAAAACGGGCTTTGAATTTGTTGAGATCGACGATGCGGACATTGTGGAAT TGAACCAGACATTAACCTTCACGGTGCCGCTGTACGAATTTTTCGCGGATTTTGCTCAAT CCTTGTTGTCCTTAGGTTGGAAACATGGCATCCATCATATTTTTGCACAAGTGGATGATG CAAATGTGAAAGGCATTATTAATCACCATCTGGGAGAGGGCGCGATTAAGCCGGCACATT ATCTGAGTTCTCTCCAGAATGGAGAGCTGCTGAAACGCAAGATGGATGAGCTTTTTGCGC GCCATCACATTAAACTTTTAGGGTATCCGACGGTCCCGTGTCGCGTGCCTCATCTGGACC ATGCAGATCGTCCGGAATTTTTTAGCCAGGCCATCCGTAACACAGACCTCGCAAGCAACG CGATGTTACCAAGCATTACAATTCCGGTCGGTCCGGAAGGCCGCCTGCCGGTCGGCCTTA GTTTTGATGCGCCTCGTGCTCGTGATGCGTTTCTCTTAAGCAATGTTAGTTTGATCGAAA AAGTGTTAAAAGGCTAATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGCAA GGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCACGC TTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCA CTCAACGC
56_premix -- 14..930 of sequence
TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGAATTTTGTCAAAATAATTTTATTGA CAACGTCTTATTAACGTTGATATAATTTAAATTTTTTTTGACAAAAATGGGCTCGTGTTG TACAATAAATGTCAGCCTGCGGTCCGGTGGCCTTAACTACCAACTCACCGGAGGGTATAT ATGTTTGGACCGGATTTCCCGTTTGCATTTGATGATTGGATCGAACATCCTAAAGGACTT GGGTCAATTCCGGCAGAAAATCACGGCGCTGAGGTTGCAATTATCGGGGCCGGCATTGCA GGCTTAGTAGCAGCATATGAATTAATGAAGATGGGTCTGAAACCTGTAGTCTATGAGGCC TCCAAAATGGGAGGTCGCTTACGGTCACAGGAATTTGAAGGAGCGAAGGGAATTGTTGCG GAACTGGGCGGTATGCGCTTTCCAGTCTCCTCCACAGCGTTCTTTCATTACGTTGATAAA CTGGGCCTTGAAAGTCGTCCGTTCCCTAACCCTTTGACGGCGGCCAGTGGCAGCACGGTG ATTGACCTGGAAGGTACCACGTACTATGCACAGATGTTATCAGATCTCCCAGCCCTGTTC CAAGAAGTGGCGGATGCATGGGCAGACGCCTTAGAATCAGGCTCTCAATTTGGAGATATT CAACAGGCCATTCGTGATTCTGACGTACCGCGTCTCAAGGAACTGTGGAACAAACTCGTG CCTTTGTGGGACGATCGCACTTTCTATGACTTTGTAGCGACCTCAAAGGCCTTTGCGAAA TTAAGCTTTTACCATCGTGAGGTTTTCGGACAAGTTGGTTTTGGTACAGGGGGTGGGATT CTGATTTTCCAATAGTA
56_premix -- 15..893 of sequence
GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCGTTTTTTTGCCGGACTGCAGCGGCC GCTACTAGTATTATTAGCCTTTTAACACTTTTTCGATCAAACTAACATTGCTTAAGAGAA ACGCATCACGAGCACGAGGCGCATCAAAACTAAGGCCGACCGGCAGGCGGCCTTCCGGAC CGACCGGAATTGTAATGCTTGGTAACATCGCGTTGCTTGCGAGGTCTGTGTTACGGATGG CCTGGCTAAAAAATTCCGGACGATCTGCATGGTCCAGATGAGGCACGCGACACGGGACCG TCGGATACCCTAAAAGTTTAATGTGATGGCGCGCAAAAAGCTCATCCATCTTGCGTTTCA GCAGCTCTCCATTCTGGAGAGAACTCAGATAATGTGCCGGCTTAATCGCGCCCTCTCCCA GATGGTGATTAATAATGCCTTTCACATTTGCATCATCCACTTGTGCAAAAATATGATGGA TGCCATGTTTCCAACCTAAGGACAACAAGGATTGAGCAAAATCCGCGAAAAATTCGTACA GCGGCACCGTGAAGGTTAATGTCTGGTTCAATTCCACAATGTCCGCATCGTCGATCTCAA CAAATTCAAAGCCCGTTTTGCGTAAGAGGCTCAGCGCATTGATACAGGCACGTTCTACTT CGCCATCTAAGTCAGACCACATGCTCACCGGCAGGCCGATGCGGCAAGGTCCCGTGCGAT TAAGAGGGAAACTCTGCTGTTTTCCTGACAGCAGCCCATACACAAATTCAGCGTCGCCAG CTGTGCGCGTCAGGAGGCCGACACAATCCTTCGTGTGGC
Sequence results to determine SpeI site problem in sfGFP
17R_premix -- 35..933 of sequence
AGCGAGTCGGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTTAAAGAA AAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGCTACTAGGT AGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGCTCTAGTATCATTACTTATACAGCT CGTCCATACCGTGGGTGATGCCCGCAGCGGTCACAAATTCCAACAGGACCATGTGATCAC GTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTGGGTGCTCAGATAGTGATTATCCGGCA GCAAAACCGGACCGTCACCAATTGGGGTGTTCTGCTGGTAATGGTCGGCCAGTTGGACGC TACCGTCCTCAACATTGTGGCGAATCTTGAAATTGGCCTTGATGCCGTTCTTTTGCTTGT CTGCCGTAATGTAAACATTATGGCTGTTGAAGTTATACTCCAGTTTATGGCCCAGAATGT TACCATCCTCTTTAAAGTCGATACCCTTCAATTCGATACGATTGACCAGCGTGTCACCTT CAAACTTAACCTCTGCGCGAGTTTTGTAGTAGCCATCATCCTTGAAGGAAATGGTACGCT CCTGGACGTAACCCTCCGGCATCGCAGATTTGAAGAAATCGTGTTGTTTCATGTGGTCCG GATAACGCGCAAAACACTGCACGCCATACGTCAACGTCGTCACCAAGGTCGGCCAAGGCA CCGGCAGTTTACCGGTGGTGCAGATGAACTTCAGGGTCAGTTTGCCGTTGGTCGCGTCGC CCTCACCTTCACCGCGAACGCTAAACTTATGACCGTTCACATCGCCGTCCAGCTCGACCA GAATCGGAACTACGCCCGTGAACAGTTCTTCGCCTTTACGCATTATCTTACCTCCTTAA
Sequence results for possible PA2652 producing colony
57.20F_premix -- 14..986 of sequence
CTATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC GGTGAACTGGCAG
57.20R_premix -- 13..1023 of sequence
ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGGAG
Sequence results for possible PA2652 producing colony
57.3F_premix -- 15..985 of sequence
TATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCT AAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGT ACAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGAC CCTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACT GTCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCG CGAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGG CAGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGC CATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTC TCAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGA TCGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGG TTCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAA ACTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTT GGACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCAC CCTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGG GTTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATG ATATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAAC TCGGTGAACTG
57.3R_premix -- 15..1019 of sequence
ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTT
Sequence results for possible PA2652 producing colony
57.7F_premix -- 14..987 of sequence
TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTA CAGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACC CTGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTG TCAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGC GAGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGC AGTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCC ATTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCT CAGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGAT CGTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGT TCCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAA CTGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTG GACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACC CTGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGG TTAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGAACTC GGTGAACTGGCAGC
57.7R_premix -- 15..1032 of sequence
GACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTT CGGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCG GCCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCA GCTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAA CCGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTT CTGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCG CATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGC TGCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCT GGATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGA CGGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCA GCAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGA CATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCAT GAATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTT GCTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCA TCTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTT CAGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCC CTGCGATCTGGCGAACTAAGCCATGAATTTTTTCAACGAAACGGTTAAAGGAGCCTGC
Sequence results for our PA2652 producing construct. Correct.
57.8F_premix -- 15..979 of sequence
TATAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCCAGCCTGCGGTCCGGTTAAAAAGAAGGGGTTCTAAATGATGCGCTTGACCC TGAAAAGTAAAGTTCTGCTGCTGGCCATGGTACCGGTGCTTTTGTTTGCACTGGTACTGT CAGGCGGCGCCGTACTGATTCTGAAAAAGCAAGCCGATGCCGAAGTCAAAGATACCCGCG AGCGCCTTTTAGGGGATCGCCGCGCGGAACTGGAGCACTACGTGCAAATCGCCATGGGCA GTATTCAAGCAGAATATGATCGCAGCGCGAACGGTGACCTCAACGCCCGCGCCGAAGCCA TTGCGCGCCTGAGCAAGATTAAATATGGAAAAGATGGTTACATTTTCGGATACGATTCTC AGGTCGTGCGGCTGTTTCGCGGGGATTCTCCTGTAGATGTAGGCAAAAGTTTTCGCGATC GTCGTGATCCGAGCGGAGTGTACTTGAATCGTGAACTTGTCGAAGCCGGACGCAACGGTT CCCACTACGTAACCTATACCTCGCCGCTGCCGGGTAACGAATCGGTGATGGTTCCTAAAC TGTCGTACACGCTGTATCTGCCGAAGTGGGATATGGTAATCGGTAGCGCGATTAATTTGG ACGGTGTCGAAGCGCAACTGGTAGAAATTAAACAGGATATTGATGAACGCATCGGCACCC TGATTGCGAGCATTGTGGGTATTGCCGGCGTACTGCTGGTAGTGCTGCTGGTGATTGGGT TAGCAGTGGCGAATGCTATGCTTCGCCCGCTTCATCAGATCCGTCAGAACCCTTGATGAT ATTGCCGCCGGCGAGGGTGATCTGACCCGCCGCCTGCCGGTGACCAGCTACGATGACTCG GTGAC
57.8R_premix -- 14..1022 of sequence
ACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTC GGTTTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGG CCGCTACTAGTATTATTAGATGCGAAACTGGCCCACCAGACGACCTAAGCCTTGACCCAG CTGCGCCAGTGACCGAGCCGTTTGTGCACCTTGCTGTGCTTCATCCGCCACTGAATCAAC CGCGCCCGCGATCTGGTGTACAGAGCGATTAATCTCTTCTGCCACTGCGGTTTGTTCTTC TGCCGCGCTCGCGATCTGCGCATTCATCGCATTAATGGTGGCAATCAGCTGGGCGATCGC ATCCAGGGAATCGCCTGCCTGATTCGCCTGATTTGAGGTTCCCTCTCCCGCTTCACCGCT GCGCCGCATGGCATCAACGGCGGCATTGGTGCCCTGTTGCAGACGATCGATCATACCCTG GATTTCCTGGGTGCTCTGCTGGGTGCGAGAAGCCAGTGCGCGCACCTCATCCGCGACGAC GGCAAATCCACGACCTGCTTCGCCTGCACGTGCTGCCTCAATGGCCGCATTCAGGGCCAG CAAATTAGTTTGTTCCGCAATGGAACGAATCACGCCCAGCACCGAGACAATGCTCTGGAC ATCTTGCTGAAGGCTATCGAGACTGGTGCCCGAATCGCGGATTTCGTCTACCAGGGCATG AATACGCTCAATAGAACCATCCACTACACGCTTAGCTGCCTGGCCTTCATGATCTGTTTG CTGAGCTGCCTCGGCTGCGCGTTGAGCGCTTTGTGCCACTTCGTGTGCGGCGGCGCTCAT CTCGTTAATCGCGGTGGCAACCTGATCGGTCTCGTGACGCTGACGTTCCATCGCTTGTTC AGAACGTTCTGCCTGCGCGCTCATTTGCTCCACTAACTGTTTCAGATCGCCCGTCATCCC TGCGATCTGGCGAACTAAGCCATGAATTTTTCAACGAAACGGTTAAAGG
Dendra2 forward sequencing results
Dendra_forward_premix -- 15..179 of sequence
TATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTCCCTATCAGTGATAGAGATTGACA TCCCTATCAGTGATAGAGATACTGAGCACTACTAGATGAACATCA
Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange
21+60a_frwrd_premix -- 15..921 of sequence
ATAATAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTA AGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGA TTATGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATC AAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAA ATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTT ACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGT TCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCG GAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACC CAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAAC TTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAA CGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAA GACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAG CTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTAC ACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGC TGATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCG CTGCAGG
Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange
21+60b_frwrd_premix -- 18..928 of sequence
TATATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC ATCGTTGAACAGTACGAACGTGCTGAAAGGTCGTCACTCCACCGGTGCTTAATAACGCTG ATAGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCT GCAGGCTTCCT
Results of J23103 ligation into RFP. Correct but Kirsten says suffix is strange
21+60c_frwrd_premix -- 18..926 of sequence
AAGATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCTGATAGCTAGCTCAGTCCTAGGGATTA TGCTAGCTACTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAAA GAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATC GAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACC AAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCC AAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAA GGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAG GACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTC CCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGT ATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGAC GGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTG CCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACC ATCGTTGAACAGTACGAACGTGCTGAAGTCGTCACTCCACCGGTGCTTAATAACGCTGAT AGTGCTAGTGTAGATCGCTACTAGAGATTAAAGAGGAGAAATACTAGTAGCGGCCGCTGC AGGCTTCCT
Results of corrected SpeI site of sfGFP
sfGFP_reverse_premix -- 20..288 of sequence
ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATCATTACTTATACAGCTCGTCCATACCGTGGGTGATGCCCGCAGCGGTCAC AAATTCCAGCAGGACCATGTGATCACGTTTTTCGTTCGGATCTTTGCTCAGCACGCTTTG GGTGCTCAGATAGTGATTATGCCGGAGCC
Sequence of Anti-holin colony 2
70.2 F_premix -- 25..943 of sequence
AAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGG ATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTACAG TGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAGCAACA GCACTTTTTGCCATGCTAGGATTGTCATTTGTTTTATCTCCATCGATTGAAGCGAATGTC GATCCTCATTTTGATAAATTTATGGAATCTGGTATTAGGCACGTTTATATGCTTTTTGAA AATAAAAGCGTAGAATCGTCTGAACAATTCTATAGTTTTATGAGAACGACCTATAAAAAT GACCCGTGCTCTTCTGATTTTGAATGTATAGAGCGAGGCGCGGAGATGGCACAATCATAC GCTAGAATTATGAACATTAAATTGGAGACTGAATAATAACCAGGCATCAAATAAAACGAA AGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCT ACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGG CCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACC GAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGT TCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATC AGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAA AAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAA TCGACGCTCAAGTCAGAGT
70.2 R_premix -- 19..1016 of sequence
GACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGT TTTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCG CTACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGA GAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTC GTTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTA TGATTGTGCCATCTCCGCGCCTCGCTCTATACATTCAAAATCAGAAGAGCACGGGTCATT TTTATAGGTCGTTCTCATAAAACTATAGAATTGTTCAGACGATTCTACGCTTTTATTTTC AAAAAGCATATAAACGTGCCTAATACCAGATTCCATAAATTTATCAAAATGAGGATCGAC ATTCGCTTCAATCGATGGAGATAAAACAAATGACAATCCTAGCATGGCAAAAAGTGCTGT TGCTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGACCGCAGGCTGGCTAGCACTG TACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCT TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTAT AGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGG CACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTT GAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATAT ACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTT GCTCAATGTACCTATAACCAGACCGTTCAGCTGGATAT
Sequence of Anti-holin colony 2
70.9 F_premix -- 18..1008 of sequence
ATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAA GGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTGACGGCTAGCTCAGTCCTAGGTAC AGTGCTAGCCAGCCTGCGGTCCGGGCGATAATAAGGAGGTACTAATGGCCTTAAAAACAA CAGCACTTTGGCACAATCATACGCTAGAATTATGAACATTAAATTGGAGACTGAATAATA ACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGT TGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTC TGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTGTCACCAC CCTGCCCTTTTTCTTTAAAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCA CTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGG TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCC CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA ACCCGGTAAGACACGACTTATCGCCACTGGC
70.9 R_premix -- 21..998 of sequence
ACGAGCGCAGCGAGTCAGTGAGCGAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTT TTAAAGAAAAAGGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGACTGCAGCGGCCGC TACTAGTATATAAACGCAGAAAGGCCCACCCGAAGGTGAGCCAGTGTGACTCTAGTAGAG AGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCG TTTTATTTGATGCCTGGTTATTATTCAGTCTCCAATTTAATGTTCATAATTCTAGCGTAT GATTGTGCCAAAGTGCTGTTGTTTTTAAGGCCATTAGTACCTCCTTATTATCGCCCGGAC CGCAGGCTGGCTAGCACTGTACCTAGGACTGAGCTAGCCGTCAACTCTAGAAGCGGCCGC GAATTCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTC GTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGT GGCACTCGAGTTGATCGGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATC ACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGA GAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTT TGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTAC GGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACAT TCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCT GGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTT TTCATCGCTCTGGAGTGA
