From 2011.igem.org
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Revision as of 01:18, 16 September 2011
Testing our designed mutants for activity on PQLP
Over 100 unique mutants were screened with a whole cell lysate assay for improved activity on the PQLP model substrate.
Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.
Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.
Our final engineered enzyme showed activity over 100 fold higher than wild type Kumamolisin, and ~700 fold higher than SC-PEP.
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