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Team:Washington/Protocols/Induc studies.
From 2011.igem.org
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# Prepare a microscope slide of each sample and Image using a microscope. | # Prepare a microscope slide of each sample and Image using a microscope. | ||
#* Pipet ~ 5 ul of each sample onto separate slides. | #* Pipet ~ 5 ul of each sample onto separate slides. | ||
| - | #* | + | #* Carefully cover the samples with agarose containing M9 + Amp + Kan + IPTG |
Latest revision as of 22:38, 15 September 2011
Induction Studies of Gene-Protein Fusions (mamK-sfGFP and mamI-sfGFP)
- Obtain a tube and fill it with 5 mL TB + Amp + Chlor.
- Obtan another tube and fill it with 1 mL TB + Amp + Chlor.
- Starting with an already prepared PBS broth, pipet 20 uL into each tube.
- Add 200 uM IPTG into each tube and incubate at room temperature.
- Prepare a microscope slide of each sample and Image using a microscope.
- Pipet ~ 5 ul of each sample onto separate slides.
- Carefully cover the samples with agarose containing M9 + Amp + Kan + IPTG
