Team:EPF-Lausanne/Our Project

From 2011.igem.org

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(Strategy)
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We want to enhance the diversity of the repressor-TF class by engineering new transcription factors that can bind unique promoter sequences different from the wild-type DNA binding site normally associated with TetR. Our proposed research plan for this summer is as follows:  
We want to enhance the diversity of the repressor-TF class by engineering new transcription factors that can bind unique promoter sequences different from the wild-type DNA binding site normally associated with TetR. Our proposed research plan for this summer is as follows:  
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'''1) introduce mutations in the TetR sequence and its binding site
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'''1) Introduce mutations in the TetR sequence and its binding site
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'''2) identify promising TF-binding site pairs (using in vivo and in vitro techniques)
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'''2) Identify promising TF-binding site pairs (using in vivo and in vitro techniques)
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'''3) conduct multiple rounds of selection to improve affinity and specificity to optimize new pairs of repressor-TFs.
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'''3) Conduct multiple rounds of selection to improve affinity and specificity to optimize new pairs of repressor-TFs.
== Strategy ==
== Strategy ==

Revision as of 18:49, 17 September 2011