Team:UT-Tokyo/Data/Method

From 2011.igem.org

(Difference between revisions)
Line 69: Line 69:
;Mg sol.
;Mg sol.
:{|
:{|
-
|MgCl2
+
|MgCl<span style="vertical-align: super;">2</span>
|500mM
|500mM
|-
|-
-
|MgSO4
+
|MgSO<span style="vertical-align: super;">4</span>
|500mM
|500mM
|}
|}

Revision as of 13:02, 15 September 2011

Reagents

  • LB broth
reagents final conc. amount
Bacto trypton 1% 1g
NaCl 0.5% 0.5g
Yeast extracs 0.5% 0.5g
MilliQ - 100ml
Total 100ml


  • SOB broth
reagents final conc. amount
Bacto trypton 2% 20g
NaCl 0.05% 0.5g
Yeast extracs 0.5% 5g
KCl (250mM) 2.5mM 10ml
MilliQ - 1000ml
Total 1L

Before use, add 10ml Mg sol.

Mg sol.
MgCl2 500mM
MgSO4 500mM


  • M9
  • 50× TAE
  • TB


Cultures

Extraction of DNA from cells

Amplification of DNA

Analysis of DNA

Assays

Retrieved from "http://2011.igem.org/Team:UT-Tokyo/Data/Method"