Team:Osaka/week7

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Contents 1 September 11(Sun) 2 September 12(Mon) 3 September 13(Tue) 4 September 14(Wed) 5 September 15(Thu) 6 September 16(Fri) 7 September 17(Sat)

September 11(Sun)

1. Gel extraction of digests
2. Miniprep of yesterday's culture
3. Restriction digests of minipreppped parts
4. Gel electrophoresis of digests
5. Ligation
   • 103(C):PprM+1-3A(vector)
   • 104(C):RecA+1-3A(vector)
   • 105(A):12(upstream)+101(downstream)+1-1G(vector)
   • 106(A):12(upstream)+102(downstream)+1-1G(vector)
6. Transformation of ligation products

September 12(Mon)

1. To liquid culture:103,104,105,106

September 13(Tue)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Ligation
   • 107(A):12(upstream)+103(downstream)+1-1G(vector)
   • 108(A):12(upstream)+104(downstream)+1-1G(vector)
5. Transformation of ligation products

September 14(Wed)

1. To liquid culture:107,108

September 15(Thu)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. To liquid culture:108

September 16(Fri)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests

September 17(Sat)

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