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Team:Lyon-INSA-ENS/Realisation/Protocols/Microscopy
From 2011.igem.org
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| - | + | <p style="text-align : center"> <font color="green" size="5"> Microscopy test</font> </p> | |
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| - | + | <p style="line-height : 1.5em"> | |
| + | This protocol aims at | ||
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| + | </p> | ||
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| - | Observe the slides with a fluorescent microscope. | + | <p style="text-align : center"> <font color="green" size="5"> Procedure </font> </p> |
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| + | <b>1.</b> In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic. | ||
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| + | <p style="line-height : 1.5em"> | ||
| + | <b>2.</b> Add 100µL of bacteria from a saturated liquid culture. | ||
| + | </p> | ||
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| + | <p style="line-height : 1.5em"> | ||
| + | <b>3.</b> Finally, add 3 or 4 sterile glass slides with a sterile tweezer. | ||
| + | </p> | ||
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| + | <p style="line-height : 1.5em"> | ||
| + | <b>4.</b> Incubate at 30°C overnight. | ||
| + | </p> | ||
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| + | <p style="line-height : 1.5em"> | ||
| + | <b>4.</b> Observe the slides with a fluorescent microscope. | ||
| + | </p> | ||
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Revision as of 11:23, 15 September 2011
Microscopy test
This protocol aims at
Procedure
1. In empty sterile plates, introduce 10mL of M63G medium and 100µL of appropriate antibiotic.
2. Add 100µL of bacteria from a saturated liquid culture.
3. Finally, add 3 or 4 sterile glass slides with a sterile tweezer.
4. Incubate at 30°C overnight.
4. Observe the slides with a fluorescent microscope.
