Team:EPF-Lausanne/Notebook/September2011

From 2011.igem.org

(Difference between revisions)
(Wednesday, 14 September 2011)
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== Wednesday, 14 September 2011 ==
== Wednesday, 14 September 2011 ==
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[[File:EPFL_Igem_1408_t7.jpg|thumb]]
 
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[[File:EPFL_Igem_1409_PlacBB.jpg|thumb]]
 
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[[File:EPFL_Igem_tetrmutants.jpg|thumb]]
 
Nadine tried to purify the PCR products of the TetR mutants, but lost them during the process... So she re-ran the PCR and used it directly do do Gibson assemblies that she later transformed into DH5alpha cells.
Nadine tried to purify the PCR products of the TetR mutants, but lost them during the process... So she re-ran the PCR and used it directly do do Gibson assemblies that she later transformed into DH5alpha cells.
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[[File:EPFL_Igem_tetrmutants.jpg|200px]]
She looked at the plates containing the biobrick variants and made colony PCRs on five colonies of variats 3,4 and 15. The resuklts show that we have 2 good colonies for 3 and one for 5.
She looked at the plates containing the biobrick variants and made colony PCRs on five colonies of variats 3,4 and 15. The resuklts show that we have 2 good colonies for 3 and one for 5.
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[[File:EPFL_Igem_1408_t7.jpg|200px]]
Finally, the Plac promoter being correctly inserted into J61002 Plac-RFP plasmid, Nadine also ran a PCR to add biobrick extensions to the promoter.
Finally, the Plac promoter being correctly inserted into J61002 Plac-RFP plasmid, Nadine also ran a PCR to add biobrick extensions to the promoter.
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[[File:EPFL_Igem_1409_PlacBB.jpg|200px]]
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{{:Team:EPF-Lausanne/Templates/Footer|title=Notebook: September 2011}}
{{:Team:EPF-Lausanne/Templates/Footer|title=Notebook: September 2011}}

Revision as of 07:17, 15 September 2011