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Team:Washington/Alkanes/Methods
From 2011.igem.org
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=Alkane Production= | =Alkane Production= | ||
| - | + | After we have the complete assembled gene in our hand, we transform it into the cell and plate the cell. At the second day, we pick the cell from the plate and inoculate them into the TB to grow overnight. | |
| + | |||
=Alkane Extraction= | =Alkane Extraction= | ||
[[File:Washington_Alkane_extraction.png|center|400px|thumb|Diagram showing the process of extraction]] | [[File:Washington_Alkane_extraction.png|center|400px|thumb|Diagram showing the process of extraction]] | ||
| - | After the cells have gone through the alkane production process, the next step is to extract alkane out of the mixture of cells and media. The method we come up here is to use acyl acetate. First, We add acyl acetate directly into the glass test tube for cell growing. Then we votex till to everything is well mixed. | + | After the cells have gone through the alkane production process, the next step is to extract alkane out of the mixture of cells and media. The method we come up here is to use acyl acetate. First, We add acyl acetate directly into the glass test tube for cell growing. Then we votex till to everything is well mixed to make sure all the alkanes go into acyl acetate. Second, we spin down the mixture by using centrifuge to form three layers (cell pallet, media, and acyl acetate), which makes it easier to take out the acyl acetate for assay. |
| + | |||
=Alkane Detection= | =Alkane Detection= | ||
Explain GCMS... again basically graphics from presentation with text | Explain GCMS... again basically graphics from presentation with text | ||
Revision as of 16:22, 15 September 2011
Basic Alkane Production Construct
Insert description and WikiDust figure here
Alkane Production
After we have the complete assembled gene in our hand, we transform it into the cell and plate the cell. At the second day, we pick the cell from the plate and inoculate them into the TB to grow overnight.
Alkane Extraction
After the cells have gone through the alkane production process, the next step is to extract alkane out of the mixture of cells and media. The method we come up here is to use acyl acetate. First, We add acyl acetate directly into the glass test tube for cell growing. Then we votex till to everything is well mixed to make sure all the alkanes go into acyl acetate. Second, we spin down the mixture by using centrifuge to form three layers (cell pallet, media, and acyl acetate), which makes it easier to take out the acyl acetate for assay.
Alkane Detection
Explain GCMS... again basically graphics from presentation with text
