Team:UT-Tokyo/LabNote

From 2011.igem.org

(Difference between revisions)
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{{:Team:UT-Tokyo/Templates/BeginContent|fullpagename=Team:UT-Tokyo/LabNote|subpagename=Lab Note}}
{{:Team:UT-Tokyo/Templates/BeginContent|fullpagename=Team:UT-Tokyo/LabNote|subpagename=Lab Note}}
 +
=Parts List=
=Parts List=
=Parts List=
{|
{|
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! Number !! Part !! Content !! Plasmid !! Length (bp)
! Number !! Part !! Content !! Plasmid !! Length (bp)
|-  
|-  
-
| #1 || J23119 || c.Promoter (Strong) || pSB1A2 || class="number" 35
+
| #1 || J23119 || c.Promoter (Strong) || pSB1A2 || class="number" |35
|-  
|-  
-
| #2 || J23118 || c.Promoter (Medium) || BBa_J61002 || class="number" 35
+
| #2 || J23118 || c.Promoter (Medium) || BBa_J61002 || class="number" |35
|-  
|-  
-
| #3 || B0032 || RBS || pSB1A2 || class="number" 13
+
| #3 || B0032 || RBS || pSB1A2 || class="number" |13
|-  
|-  
-
| #5 || B0014 || d.Ter || pSB1AK3 || class="number" 95
+
| #5 || B0014 || d.Ter || pSB1AK3 || class="number" |95
|-  
|-  
-
| #9 || E0240 || RBS-GFP-d.Ter || pSB1A2 || class="number" 876
+
| #9 || E0240 || RBS-GFP-d.Ter || pSB1A2 || class="number" |876
|-  
|-  
-
| #10 || E0040 || GFP || pSB1A2 || class="number" 681
+
| #10 || E0040 || GFP || pSB1A2 || class="number" |681
|-  
|-  
-
| #11 || E1010 || RFP || pSB2K3 || class="number" 723
+
| #11 || E1010 || RFP || pSB2K3 || class="number" |723
|-  
|-  
-
| #14 || I712019 || fLuc || pSB1AK8 || class="number" 1653
+
| #14 || I712019 || fLuc || pSB1AK8 || class="number" |1653
|-  
|-  
-
| #17 || J52008 || rLuc || pSB1AK3 || class="number" 936
+
| #17 || J52008 || rLuc || pSB1AK3 || class="number" |936
|-  
|-  
-
| #20 || R0011 || lacP || pSB1A2 || class="number" 55
+
| #20 || R0011 || lacP || pSB1A2 || class="number" |55
|-  
|-  
-
| #21 || C0012 || LacI || pSB1A2 || class="number" 1128
+
| #21 || C0012 || LacI || pSB1A2 || class="number" |1128
|-  
|-  
-
| #22 || I712074 || pT7 || pSB1AK8 || class="number" 46
+
| #22 || I712074 || pT7 || pSB1AK8 || class="number" |46
|-  
|-  
-
| #23 || K145001 || T7 RNA Pol. || pSB1A2 || class="number" 2655
+
| #23 || K145001 || T7 RNA Pol. || pSB1A2 || class="number" |2655
|-  
|-  
-
| #24 || J22106 || recAp || pSB1A2 || class="number" 192
+
| #24 || J22106 || recAp || pSB1A2 || class="number" |192
|-  
|-  
-
| #27 || C0083 || AspA || pSB2K3 || class="number" 1518
+
| #27 || C0083 || AspA || pSB2K3 || class="number" |1518
|-  
|-  
-
| #28 || K112808 || T4 phage lysis device (no promoter) || pSB1A2 || class="number" 1785
+
| #28 || K112808 || T4 phage lysis device (no promoter) || pSB1A2 || class="number" | 1785
|-  
|-  
-
| #29 || - || CheZ || pSB1AK3 || class="number" 728
+
| #29 || - || CheZ || pSB1AK3 || class="number" | 728
|-  
|-  
-
| #30 || K117000 || Lysis gene || pSB1A2 || class="number" 144
+
| #30 || K117000 || Lysis gene || pSB1A2 || class="number" | 144
|-  
|-  
-
| #31 || - || LexA || pSB1AK3 || class="number" 750
+
| #31 || - || LexA || pSB1AK3 || class="number" | 750
|-  
|-  
-
| #33 || - || sulAp || pSB1AK3 || class="number" 67
+
| #33 || - || sulAp || pSB1AK3 || class="number" | 67
|-  
|-  
-
| #34 || - || uvrAp || pSB1AK3 || class="number" 96
+
| #34 || - || uvrAp || pSB1AK3 || class="number" | 96
|-  
|-  
| #35 || - || recNp ||  ||  
| #35 || - || recNp ||  ||  
|-  
|-  
-
| #36 || R0051 || cI-repressed promoter || pSB1A2 || class="number" 49
+
| #36 || R0051 || cI-repressed promoter || pSB1A2 || class="number" | 49
|-  
|-  
| #37 || C0051 || cI repressor (LVA tagged) || pSB1A2 || \
| #37 || C0051 || cI repressor (LVA tagged) || pSB1A2 || \
Line 59: Line 60:
|}
|}
<!--
<!--
-
| #2-9 ||  || c.Promoter (M)-RBS-GFP-d.Ter || pSB1A2 || class="number" 911
+
| #2-9 ||  || c.Promoter (M)-RBS-GFP-d.Ter || pSB1A2 || class="number" | 911
|-  
|-  
-
| #20-9 ||  || lacP-RBS-GFP-d.Ter || pSB1A2 || class="number" 931
+
| #20-9 ||  || lacP-RBS-GFP-d.Ter || pSB1A2 || class="number" | 931
|-  
|-  
-
| #22-9 ||  || pT7-RBS-GFP-d.Ter || pSB1AK8 || class="number" 922
+
| #22-9 ||  || pT7-RBS-GFP-d.Ter || pSB1AK8 || class="number" | 922
|-  
|-  
-
| #3-11 ||  || RBS-RFP || pSB1A2 || class="number" 736
+
| #3-11 ||  || RBS-RFP || pSB1A2 || class="number" | 736
|-  
|-  
-
| #3-11-5 ||  || RBS-RFP-d.Ter || pSB1AK3 || class="number" 831
+
| #3-11-5 ||  || RBS-RFP-d.Ter || pSB1AK3 || class="number" | 831
|-  
|-  
-
|  ||  ||  || pSB1C3 || class="number" 831
+
|  ||  ||  || pSB1C3 || class="number" | 831
|-  
|-  
-
| #2-3-11-5 ||  || c.Promoter(M)-RBS-RFP-d.Ter || pSB1A2 || class="number" 866
+
| #2-3-11-5 ||  || c.Promoter(M)-RBS-RFP-d.Ter || pSB1A2 || class="number" | 866
|-  
|-  
-
|  ||  ||  || pSB1AK3 || class="number" 866
+
|  ||  ||  || pSB1AK3 || class="number" | 866
|-  
|-  
-
| #24-3-11-5 ||  || recAp-RBS-RFP-d.Ter || pSB1A2 || class="number" 1023
+
| #24-3-11-5 ||  || recAp-RBS-RFP-d.Ter || pSB1A2 || class="number" | 1023
|-  
|-  
-
| #33-3-11-5 ||  || sulAp-RBS-RFP-d.Ter || pSB1AK3 || class="number" 898
+
| #33-3-11-5 ||  || sulAp-RBS-RFP-d.Ter || pSB1AK3 || class="number" | 898
|-  
|-  
-
| #14-5 ||  || fLuc-d.Ter || pSB1AK3 || class="number" 1748
+
| #14-5 ||  || fLuc-d.Ter || pSB1AK3 || class="number" | 1748
|-  
|-  
-
| #3-14-5 ||  || RBS-ｆLuc-d.Ter || pSB1A2 || class="number" 1761
+
| #3-14-5 ||  || RBS-ｆLuc-d.Ter || pSB1A2 || class="number" | 1761
|-  
|-  
-
| #2-3-14-5 ||  || c.Promoter (M)-RBS-fLuc-d.Ter  || pSB1A2 || class="number" 1796
+
| #2-3-14-5 ||  || c.Promoter (M)-RBS-fLuc-d.Ter  || pSB1A2 || class="number" | 1796
|-  
|-  
-
| #20-3-14-5 ||  || lacP-RBS-fLuc-d.Ter || pSB1A2 || class="number" 1816
+
| #20-3-14-5 ||  || lacP-RBS-fLuc-d.Ter || pSB1A2 || class="number" | 1816
|-  
|-  
-
| #34-3-14-5 ||  || uvrAp-RBS-fLuc-d.Ter || pSB1AK3 || class="number" 1857
+
| #34-3-14-5 ||  || uvrAp-RBS-fLuc-d.Ter || pSB1AK3 || class="number" | 1857
|-  
|-  
-
| #36-3-14-5 ||  || cIp-RBS-fLuc-d.Ter || pSB1A2 || class="number" 1810
+
| #36-3-14-5 ||  || cIp-RBS-fLuc-d.Ter || pSB1A2 || class="number" | 1810
|-  
|-  
-
| #17-5 ||  || ｒLuc-d.Ter  || pSB1AK3 || class="number" 1031
+
| #17-5 ||  || ｒLuc-d.Ter  || pSB1AK3 || class="number" | 1031
|-  
|-  
-
| #3-17-5 ||  || RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" 1044
+
| #3-17-5 ||  || RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" | 1044
|-  
|-  
-
| #2-3-17-5 ||  || c.Promoter (M)-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" 1079
+
| #2-3-17-5 ||  || c.Promoter (M)-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" | 1079
|-  
|-  
-
| #24-3-17-5 ||  || recAp-RBS-rLuc-d.Ter || pSB1A2 || class="number" 1236
+
| #24-3-17-5 ||  || recAp-RBS-rLuc-d.Ter || pSB1A2 || class="number" | 1236
|-  
|-  
-
| #34-3-17-5 ||  || uvrAp-RBS-rLuc-d.Ter || pSB1AK3 || class="number" 1140
+
| #34-3-17-5 ||  || uvrAp-RBS-rLuc-d.Ter || pSB1AK3 || class="number" | 1140
|-  
|-  
-
| #20-3-17-5 ||  || lacP-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" 1099
+
| #20-3-17-5 ||  || lacP-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" | 1099
|-  
|-  
-
| #3-14-5-2-3-17-5 ||  || RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" 2840
+
| #3-14-5-2-3-17-5 ||  || RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" | 2840
|-  
|-  
-
| #20-3-14-5-2-3-17-5 ||  || lacP-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" 2895
+
| #20-3-14-5-2-3-17-5 ||  || lacP-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" | 2895
|-  
|-  
-
| #36-3-14-5-2-3-17-5 ||  || cIp-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" 2889
+
| #36-3-14-5-2-3-17-5 ||  || cIp-RBS-ｆLuc-d.Ter-c.Promoter (M)-RBS-ｒLuc-d.Ter  || pSB1A2 || class="number" | 2889
|-  
|-  
-
| #3-27 ||  || RBS-AspA || pSB1A2 || class="number" 1531
+
| #3-27 ||  || RBS-AspA || pSB1A2 || class="number" | 1531
|-  
|-  
-
| #3-27-5 ||  || RBS-AspA-d.Ter || pSB1AK3 || class="number" 1626
+
| #3-27-5 ||  || RBS-AspA-d.Ter || pSB1AK3 || class="number" | 1626
|-  
|-  
-
| #20-3-27-5 ||  || lacP-RBS-AspA-d.Ter || pSB1A2 || class="number" 1681
+
| #20-3-27-5 ||  || lacP-RBS-AspA-d.Ter || pSB1A2 || class="number" | 1681
|-  
|-  
-
| #3-29 ||  || RBS-CheZ || pSB1A2 || class="number" 741
+
| #3-29 ||  || RBS-CheZ || pSB1A2 || class="number" | 741
|-  
|-  
-
| #3-29-5 ||  || RBS-CheZ-d.Ter || pSB1AK3 || class="number" 836
+
| #3-29-5 ||  || RBS-CheZ-d.Ter || pSB1AK3 || class="number" | 836
|-  
|-  
-
| #20-3-29-5 ||  || lacP-RBS-CheZ-d.Ter  || pSB1A2 || class="number" 891
+
| #20-3-29-5 ||  || lacP-RBS-CheZ-d.Ter  || pSB1A2 || class="number" | 891
|-  
|-  
-
| #36-3-29-5 ||  || cIp-RBS-CheZ-d.Ter || pSB1A2 || class="number" 885
+
| #36-3-29-5 ||  || cIp-RBS-CheZ-d.Ter || pSB1A2 || class="number" | 885
|-  
|-  
| #37-5 ||  || cI-d.Ter || pSB1AK3 || #VALUE!
| #37-5 ||  || cI-d.Ter || pSB1AK3 || #VALUE!
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| #20-3-37-5 ||  || lacP-RBS-cI-d.Ter || pSB1A2 || #VALUE!
| #20-3-37-5 ||  || lacP-RBS-cI-d.Ter || pSB1A2 || #VALUE!
|-  
|-  
-
| #20-28 ||  || lacP-〈T4 phage lysis device〉 || pSB1A2 || class="number" 1840
+
| #20-28 ||  || lacP-〈T4 phage lysis device〉 || pSB1A2 || class="number" | 1840
|-  
|-  
-
| #3-30 ||  || RBS-Lysis gene || pSB1A2 || class="number" 157
+
| #3-30 ||  || RBS-Lysis gene || pSB1A2 || class="number" | 157
|-  
|-  
-
| #3-23 ||  || RBS-T7 RNA Pol. || pSB1A2 || class="number" 2668
+
| #3-23 ||  || RBS-T7 RNA Pol. || pSB1A2 || class="number" | 2668
|-  
|-  
-
| pSB1A2 ||  || Plasmid backbone || pSB1A2 || class="number" 2079
+
| pSB1A2 ||  || Plasmid backbone || pSB1A2 || class="number" | 2079
|-  
|-  
-
| pSB1AK3 ||  || Plasmid backbone || pSB1AK3 || class="number" 3189
+
| pSB1AK3 ||  || Plasmid backbone || pSB1AK3 || class="number" | 3189
|-  
|-  
-
| pSB1A3 ||  || Linearized plasmid backbone || pSB1A3 || class="number" 2155
+
| pSB1A3 ||  || Linearized plasmid backbone || pSB1A3 || class="number" | 2155
|-  
|-  
-
| pSB1K3 ||  || Linearized plasmid backbone || pSB1K3 || class="number" 2204
+
| pSB1K3 ||  || Linearized plasmid backbone || pSB1K3 || class="number" | 2204
|-  
|-  
-
| pSB1C3 ||  || Linearized plasmid backbone || pSB1C3 || class="number" 2070
+
| pSB1C3 ||  || Linearized plasmid backbone || pSB1C3 || class="number" | 2070
|-  
|-  
-
| pSB1C3 || J04450 || lacP-RBS-RFP-d.Ter || pSB1C3 || class="number" 1069
+
| pSB1C3 || J04450 || lacP-RBS-RFP-d.Ter || pSB1C3 || class="number" | 1069
|}
|}
-->
-->
 +

Revision as of 20:53, 13 September 2011

Parts List

Parts List

Table 1. List of iGEM parts
Number Part Content Plasmid Length (bp)
#1 J23119 c.Promoter (Strong) pSB1A2 35
#2 J23118 c.Promoter (Medium) BBa_J61002 35
#3 B0032 RBS pSB1A2 13
#5 B0014 d.Ter pSB1AK3 95
#9 E0240 RBS-GFP-d.Ter pSB1A2 876
#10 E0040 GFP pSB1A2 681
#11 E1010 RFP pSB2K3 723
#14 I712019 fLuc pSB1AK8 1653
#17 J52008 rLuc pSB1AK3 936
#20 R0011 lacP pSB1A2 55
#21 C0012 LacI pSB1A2 1128
#22 I712074 pT7 pSB1AK8 46
#23 K145001 T7 RNA Pol. pSB1A2 2655
#24 J22106 recAp pSB1A2 192
#27 C0083 AspA pSB2K3 1518
#28 K112808 T4 phage lysis device (no promoter) pSB1A2 1785
#29 - CheZ pSB1AK3 728
#30 K117000 Lysis gene pSB1A2 144
#31 - LexA pSB1AK3 750
#33 - sulAp pSB1AK3 67
#34 - uvrAp pSB1AK3 96
#35 - recNp
#36 R0051 cI-repressed promoter pSB1A2 49
#37 C0051 cI repressor (LVA tagged) pSB1A2 \
#38 - RecA


Lab Diary

For convenience sake, each part (gene, promotors, etc) are represented by consecutive numbers (e.g. #20 for lac promotor). By pointing the mouse on the part number in the labnote, you can find out the details of the part.


Retrieved from "http://2011.igem.org/Team:UT-Tokyo/LabNote"