Team:EPF-Lausanne/Notebook/September2011

From 2011.igem.org

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(Someday, 12 September 2011)
(Someday, 12 September 2011)
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Nadine started liquid cultures for tomorrow's platereader experiment. She also made the PCRs for putting TetR mutants into the pSB3K1 Pconst-TetR plasmid by Gibson. We can see the expected band at around 700 bp!
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Nadine started liquid cultures for tomorrow's platereader experiment. She also made the PCRs for putting TetR mutants into the pSB3K1 Pconst-TetR plasmid by Gibson. We can see the expected band at around 700 bp! Unfortunately, amplification of the pSB3K1 backbone didn't work...
[[File:EPFL_Igem_tetrmutants_1209.jpg|thumb]]
[[File:EPFL_Igem_tetrmutants_1209.jpg|thumb]]

Revision as of 09:22, 13 September 2011