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Team:EPF-Lausanne/Our Project/T7 promoter variants
From 2011.igem.org
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(Created page with "{{:Team:EPF-Lausanne/Templates/Header|title=Microfluidics-work in progress}} to write here: why we wanted to make these T7 variants, how we did it (explain the multiple rounds ...") |
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| - | + | A good way to characterize promoter strength is to use a fluorescence gene that is driven by the promoter. The fluorescence induction curves reveal both the speed and strength of the promoter. | |
| - | {{:Team:EPF-Lausanne/Templates/Footer}} | + | To add an extra degree of control to our plasmid system, we wanted to be able to influence the strength of the promoter that induces lysis or RFP. To that end, we manufactured a family of T7 promoter variants that we characterized using RFP fluorescence. |
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| + | {{:Team:EPF-Lausanne/Templates/Footer}} | ||
Revision as of 14:57, 14 September 2011
Microfluidics-work in progress
A good way to characterize promoter strength is to use a fluorescence gene that is driven by the promoter. The fluorescence induction curves reveal both the speed and strength of the promoter.
To add an extra degree of control to our plasmid system, we wanted to be able to influence the strength of the promoter that induces lysis or RFP. To that end, we manufactured a family of T7 promoter variants that we characterized using RFP fluorescence.
