Revision as of 11:40, 12 September 2011

Team IGEM Paris 2011

Parameters

Description	Value	Unit	Reference
Concentration of X	NA	molecules per cell	Notation convention
Delay due to protein X production and maturation	NA	s	Notation convention
Maximal production rate of pVeg promoter (constitutive)	0.02	molecules.s^-1 or pops	Estimated, see the justification
Maximal production rate of pLac promoter	0.02	molecules.s^-1 or pops	Estimated, see the justification
Maximal production rate of pT7 promoter	0.02	molecules.s^-1 or pops	Estimated, see the justification
Dissociation constant for IPTG to LacI	1200	molecules per cell	Aberdeen 2009 wiki
Dissociation constant for LacI to LacO (pLac)	700	molecules per cell	Aberdeen 2009 wiki
Dissociation constant for T7 RNA polymerase to pT7	10	molecules per cell	We used the classic assumption 1nM=1 molecule per cell and [1]
Translation rate of proteins	0.9	s^-1	Estimated, see the justification
Dilution rate in exponential phase	2.88x10^-4	s^-1	Calculated with a 40 min generation time. See explanation
Degradation rate of mRNA	2.88x10^-3	s^-1	Uri Alon (To Be Confirmed)
Degradation rate of GFP	10^-4	s^-1	BioNumbers
Degradation rate of RFP	10^-4	s^-1	Estimated equal to GFP degradation rate
Delay due tT7 RNA polymerase production and maturation	300	s	[2]
Delay due GFP production and maturation	360	s	BioNumbers
Delay due RFP production and maturation	360	s	Estimated equal to GFP delay (similar molecules)
Delay due to mRNA production	30	s	BioNumbers with an approximation: all our contructs are around 1-2kb

References

Cytoplasmic expression of a reporter gene by co-delivery of T7 RNA polymerase and T7 promoter sequence with cationic liposomes, X Gao and L Huang, accessible here
Molecular Biology for Masters by Dr. G. R. Kantharaj, accessible here

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