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Team:Paris Bettencourt/GFPLac diffusion
From 2011.igem.org
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{{:Team:Paris_Bettencourt/tpl_test}} | {{:Team:Paris_Bettencourt/tpl_test}} | ||
=The YFP Concentration design= | =The YFP Concentration design= | ||
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| + | YFP:tetR is a recombinant fusion protein composed by 1357 bp | ||
| + | Their origin come from François-Xavier Barre, Andrew Wright and Dave Lane ( | ||
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| + | == Making the YFP:tetR diffuse through the tube == | ||
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| + | In the Ben-Yehuda paper, GFP has been proved to pass though the nanotubes. We start to build the same experiment but improved by the tetR:YFP - tetO Array system.<br> | ||
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| + | [[span style="color:blue"]]In the emittor cell[[/span]], we have to over express the T7 polymerase for them to have a chance to pass through the tube. As we said in the general overview the production of T7 polymsease is over the control of an IPTG inducible promoter design to have a slow response by the over-expression of LacI in the cell. The RFP, placed on the same mRNA, is behaving like a reporter of the quantity of the produced T7 polymerase.<br> | ||
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| + | [[span style="color:blue"]]In the receiver cell[[/span]], a system, sensitive to the T7 polymerase will be activated if one T7 polymerase reach on of its promoter, present in a few plasmids of the receiver cell (low copy). The system is self amplifying and the GFP is produced as a monitor of the signal. | ||
| + | The principle of the design is summed up in the image below | ||
[[File:yfptetrdesign2.jpg|703px|thumb|center|the YFP:tetR design]] | [[File:yfptetrdesign2.jpg|703px|thumb|center|the YFP:tetR design]] | ||
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| + | == Model and experiments == | ||
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Revision as of 15:57, 11 September 2011
The YFP Concentration design
YFP:tetR is a recombinant fusion protein composed by 1357 bp Their origin come from François-Xavier Barre, Andrew Wright and Dave Lane (
Making the YFP:tetR diffuse through the tube
In the Ben-Yehuda paper, GFP has been proved to pass though the nanotubes. We start to build the same experiment but improved by the tetR:YFP - tetO Array system.
span style="color:blue"In the emittor cell/span, we have to over express the T7 polymerase for them to have a chance to pass through the tube. As we said in the general overview the production of T7 polymsease is over the control of an IPTG inducible promoter design to have a slow response by the over-expression of LacI in the cell. The RFP, placed on the same mRNA, is behaving like a reporter of the quantity of the produced T7 polymerase.
span style="color:blue"In the receiver cell/span, a system, sensitive to the T7 polymerase will be activated if one T7 polymerase reach on of its promoter, present in a few plasmids of the receiver cell (low copy). The system is self amplifying and the GFP is produced as a monitor of the signal. The principle of the design is summed up in the image below
Model and experiments
