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Team:Paris Liliane Bettencourt/Notebook/2011/09/08/
From 2011.igem.org
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[[File:CP0909_T7ter.jpg|thumb|T7 ter cutted]] | [[File:CP0909_T7ter.jpg|thumb|T7 ter cutted]] | ||
[[File:CP0909_KinAter.jpg|thumb|KinA ter cutted]] | [[File:CP0909_KinAter.jpg|thumb|KinA ter cutted]] | ||
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Then I proceed to a gel extraction of the bands. | Then I proceed to a gel extraction of the bands. | ||
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Runned on a gel | Runned on a gel | ||
| + | [[File:CP0909_TetRTetO.jpg|thumb|PCR colony 2,4,5,6,7,8,9,10]] | ||
=== Miniprep of YFP-TetR BB === | === Miniprep of YFP-TetR BB === | ||
Miniprep of the 3 last clones of YFP-TetR BB (that were not sequenced or red) | Miniprep of the 3 last clones of YFP-TetR BB (that were not sequenced or red) | ||
Revision as of 08:27, 9 September 2011
Contents |
Cyrille
Digestion and gel
S24 is prepared to be cloned with
- RFP - TT
- T7 amber - TT
- ComS
- KinA-TT
3*4 500ng of S24 was digested and runned on the gel The insert was digested several time.
Then the gel was runned and the bands cutted.
Then I proceed to a gel extraction of the bands.
PCR colony of TetO/TetR
New attempt of PCR colony to find one good clone. Runned on a gel
Miniprep of YFP-TetR BB
Miniprep of the 3 last clones of YFP-TetR BB (that were not sequenced or red)
