Team:Paris Liliane Bettencourt/Notebook/2011/09/08/

From 2011.igem.org

(Difference between revisions)
(Created page with "{{:Team:Paris_Bettencourt/tpl_test}} == Cyrille == === Digestion and gel === S24 is prepared to be cloned with * RFP - TT * T7 amber - TT * ComS * KinA-TT")
(→Digestion and gel)
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* ComS
* ComS
* KinA-TT
* KinA-TT
 +
 +
3*4 500ng of S24 was digested and runned on the gel
 +
The insert was digested several time
 +
 +
=== PCR colony of TetO/TetR ===
 +
 +
New attempt of PCR colony to find one good clone.
 +
Runned on a gel
 +
 +
 +
=== Miniprep of YFP-TetR BB ===
 +
 +
Miniprep of the 3 last clones of YFP-TetR BB (that were not sequenced or red)

Revision as of 15:48, 8 September 2011

Team IGEM Paris 2011

Contents

Cyrille

Digestion and gel

S24 is prepared to be cloned with

  • RFP - TT
  • T7 amber - TT
  • ComS
  • KinA-TT

3*4 500ng of S24 was digested and runned on the gel The insert was digested several time

PCR colony of TetO/TetR

New attempt of PCR colony to find one good clone. Runned on a gel


Miniprep of YFP-TetR BB

Miniprep of the 3 last clones of YFP-TetR BB (that were not sequenced or red)

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