Team:EPF-Lausanne/Notebook/September2011

From 2011.igem.org

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(Tuesday, 6 September 2011)
(Wednesday, 7 September 2011)
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Nadine made colony PCRs for the T7 promoter variants that Henrike cloned into the biobrick vector. She also prepared some liquid cultures for tomorrow's platereader experiment.
Nadine made colony PCRs for the T7 promoter variants that Henrike cloned into the biobrick vector. She also prepared some liquid cultures for tomorrow's platereader experiment.
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Vdog made glycerol stocks and minipreps of the non-randomer RFP variants 6, 13, and 14 as well as Alina's T7-const-C2/C11, T7 lac 1, and T7 lac2 3.
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He also sent all of Alina's constructs (aforementioned) for sequencing using two different primers: 947_f and 816_r. He then ran a control PCR on all the T7 constructs using Alina's EPFL TAQ with the following combination of primers:
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* 440-f and 1333-r
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* 947-f and pSB3K1-ext-r
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The protocol asked for a 25 uL aliquot with
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2.5 uL Thermo Pol buffer (TPB)
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.5 uL dNTP
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.5 uL primer 1
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.5 uL primer 2
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1 uL plasmid
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.25 uL TAQ
{{:Team:EPF-Lausanne/Templates/Footer|title=Notebook: September 2011}}
{{:Team:EPF-Lausanne/Templates/Footer|title=Notebook: September 2011}}

Revision as of 07:14, 8 September 2011