Team:Edinburgh/Data
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- | : a LacZα part with a BglII restriction site at the start; useful for cloning new parts via PCR using Blue/White selection. | + | : a LacZα part with a BglII restriction site at the start; useful for cloning new parts via PCR using Blue/White selection, as well as being a BioSandwich-compatible promoter. |
; <partinfo>BBa_K523013</partinfo> | ; <partinfo>BBa_K523013</partinfo> |
Revision as of 13:09, 6 September 2011
Data Overview
The iGEM rules require us to have simple illustrations of how our devices work and where the Parts function in the system; and links to the Registry for the parts/constructs for which we have produced data.
See the sample Data page.
Contents |
Overview
This page provides an overview of the purely biological aspects of the project.
Cell Surface Display System
(Further details are at the dedicated Cell Surface Display page.)
This system aims at achieving synergy between the enzymes by displaying them at high copy number on the cell's outer membrane. Ice Nucleation Protein is used as a carrier for display of the enzymes; it carries them to the outer membrane.
Schematic diagram
Phage Display System
(Further details are at the dedicated Phage Display page.)
This system aims at achieving synergy between the enzymes by displaying them on an M13 phage. The major coat protein pVIII is used as a carrier for display of the enzymes; it incorporates them into the phage.
Schematic diagram
BioSandwich assembly
To do...
Favourite BioBricks
(A complete list of parts made during the project is found at the dedicated Parts page.)
- <partinfo>BBa_K523000</partinfo>
- a LacZα part with a BglII restriction site at the start; useful for cloning new parts via PCR using Blue/White selection, as well as being a BioSandwich-compatible promoter.
- <partinfo>BBa_K523013</partinfo>
- a fusion of Ice Nucleation Protein and Enhanced Yellow Fluorescent Protein under the control of the Lac promoter.