Team:Groningen/project AND gate

From 2011.igem.org

(Difference between revisions)
(→AND gate)
(→AND gate)
Line 2: Line 2:
=AND gate=
=AND gate=
-
In order to integrate the memory units, we designed the AND gate system. To modulate gene expression at the translation level, we harness the use of engineered RNA-based/riboregulator system. After transcription, the 5’ UTR of crRNA-LasR transcript will form a stem-loop structure that blocks the RBS and prevents translation. To activate the expression of a self-repressed gene, the taRNA which is complementary to crRNA will unfold the stem-loop structure thus exposing the obstructed RBS and permit translation. (Isaacs, 2006)
+
In order to integrate the memory units, we designed an AND gate system. To modulate gene expression on the translational level, we utilized an engineered riboregulator system introduced by Isaacs, et al. in 2004. Upon transcription the 5’ UTR of the crRNA-LasR transcript will form a stem-loop structure subsequently blocking the RBS and preventing translation. Transcription of a taRNA which is partly complementary to crRNA will unfold the stem loop structure thus exposing the obstructed RBS and permitting translation.  
-
 
+
-
==Parts==
+
-
The design of the AND gate circuit is based on two constructs, noncoding taRNA-DT cloned downstream of a hybB promoter (PhybB) and a cI promoter (PcI) cloned upstream crRNA-RBS-LasR-TAG-DT. In attempt to characterize the functional assembled parts, we cotransformed plasmid harboring AND gate system with reporter plasmid downstream LasR promoter.
+
{{FooterGroningen2011}}
{{FooterGroningen2011}}

Revision as of 17:41, 6 September 2011


AND gate

In order to integrate the memory units, we designed an AND gate system. To modulate gene expression on the translational level, we utilized an engineered riboregulator system introduced by Isaacs, et al. in 2004. Upon transcription the 5’ UTR of the crRNA-LasR transcript will form a stem-loop structure subsequently blocking the RBS and preventing translation. Transcription of a taRNA which is partly complementary to crRNA will unfold the stem loop structure thus exposing the obstructed RBS and permitting translation.




Retrieved from "http://2011.igem.org/Team:Groningen/project_AND_gate"