"
Team:Paris Liliane Bettencourt/Notebook/2011/09/05/
From 2011.igem.org
(Difference between revisions)
(Created page with "{{:Team:Paris_Bettencourt/tpl_test}} == Cyrille == === Miniprep === Miniprep of 2 clones of TetO array in pSB1C3, 2 clones of TetO array in pHM3 Miniprep of Mathias: S21 S82 1...") |
(→Ligation) |
||
| Line 15: | Line 15: | ||
=== Ligation === | === Ligation === | ||
| + | |||
| + | Ligation of pHM3 Digested EP with pVeg-YFP-TetR D. EP with ratio 1:1 1:10 1:100 | ||
| + | Ligation pHM3 TetO D. SP with pVeg-YFP-TetR D. SP | ||
| + | |||
| + | === Transformation === | ||
| + | |||
| + | Transformation of the ligation product | ||
| + | + ComS ligated in pSB1C3 | ||
| + | + YFP TetR BB ligated in pSB1C3 | ||
Revision as of 13:07, 6 September 2011
Contents |
Cyrille
Miniprep
Miniprep of 2 clones of TetO array in pSB1C3, 2 clones of TetO array in pHM3 Miniprep of Mathias: S21 S82 1 2 3 4; S75 S67 and S75
With good yeilds (>500ng)
Gel Extraction
Gel extraction of pHM3 Digested in EP and pVeg YFP TetR D EP and DXP.
Ligation
Ligation of pHM3 Digested EP with pVeg-YFP-TetR D. EP with ratio 1:1 1:10 1:100 Ligation pHM3 TetO D. SP with pVeg-YFP-TetR D. SP
Transformation
Transformation of the ligation product + ComS ligated in pSB1C3 + YFP TetR BB ligated in pSB1C3
