Team:Valencia/Parts
From 2011.igem.org
(Prototype team page) |
|||
(16 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | < | + | <html><link rel="stylesheet" href="https://2011.igem.org/wiki/index.php?title=User:Arnau/valencia.css&action=raw&ctype=text/css" type="text/css"></html> |
- | + | {{Template:Valencia-0-3}} | |
<html> | <html> | ||
- | < | + | <body> |
- | + | <style type="text/css"> | |
- | + | .center { | |
- | + | width:980px; | |
- | + | height:auto; | |
- | + | margin-left:px; | |
- | </ | + | margin-right:px; |
- | < | + | margin-top:20px; |
- | + | margin-bottom:0px; | |
- | + | } | |
- | + | ||
- | + | .col_center{ | |
+ | width:970px; | ||
+ | height:auto; | ||
+ | float:left; | ||
+ | background-color:transparent; | ||
+ | |||
+ | } | ||
+ | |||
+ | .col_center_top{ | ||
+ | width:908px; | ||
+ | background-color:#0087ba; | ||
+ | height:20px; | ||
+ | font-family:Verdana, Arial, Helvetica, sans-serif; | ||
+ | font-size:20px; | ||
+ | color:#fff; | ||
+ | padding:20px; | ||
+ | float:center; | ||
+ | margin-right:15px; | ||
+ | |||
+ | } | ||
+ | |||
+ | .col_center_center{ | ||
+ | width:850px; | ||
+ | text-align:justify; | ||
+ | height: auto; | ||
+ | font-family:Verdana, Arial, Helvetica, sans-serif; | ||
+ | font-size:14px; | ||
+ | color:black; | ||
+ | padding:20px; | ||
+ | } | ||
+ | |||
+ | .col_center_bottom{ | ||
+ | width:900px; | ||
+ | text-align:center; | ||
+ | height: auto; | ||
+ | font-family:Verdana, Arial, Helvetica, sans-serif; | ||
+ | font-size:14px; | ||
+ | color:#fff; | ||
+ | padding:20px; | ||
+ | } | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | html, body { | ||
+ | margin: 0; | ||
+ | padding: 0; | ||
+ | } | ||
+ | |||
+ | body { | ||
+ | background:darkblue url(https://static.igem.org/mediawiki/2011/6/65/Valencia_Aguamitadinferior1500x800.jpg) repeat-x top fixed; | ||
+ | |||
+ | } | ||
+ | |||
+ | #globalWrapper { background-color: transparent; border: none; margin: 0; padding: 0;} | ||
+ | #content{ | ||
+ | background:transparent url("https://static.igem.org/mediawiki/2011/f/fd/Valencia_Transpa30a.png") center fixed repeat-y; | ||
+ | padding: 0px; | ||
+ | margin:0 auto; | ||
+ | border: none; | ||
+ | |||
+ | |||
+ | } | ||
+ | |||
+ | </style> | ||
+ | <script type="text/javascript" | ||
+ | src="http://ajax.googleapis.com/ajax/libs/jquery/1.3/jquery.min.js"></script> | ||
+ | <script type="text/javascript"> | ||
+ | $(document).ready(function() { | ||
+ | $("ul#topnav li").hover(function() { | ||
+ | $(this).css({ 'background' : 'transparent url(https://static.igem.org/mediawiki/2010/0/03/Valencia_navbar_active.gif) repeat-x'}); | ||
+ | $(this).find("span").show(); | ||
+ | } , function() { //on hover out... | ||
+ | $(this).css({ 'background' : 'none'}); | ||
+ | $(this).find("span").hide(); | ||
+ | }); | ||
+ | |||
+ | }); | ||
+ | </script> | ||
+ | |||
+ | <style type="text/css"> | ||
+ | |||
+ | #headerlinks{ | ||
+ | margin-left: -12px; | ||
+ | } | ||
+ | |||
+ | #nav, #nav ul { | ||
+ | padding: 0; | ||
+ | list-style: none; | ||
+ | } | ||
+ | |||
+ | .abstractext{ | ||
+ | float:right; | ||
+ | width="400px" | ||
+ | } | ||
+ | |||
+ | #nav a { | ||
+ | display: block; | ||
+ | width: 149px; | ||
+ | height: 40px; | ||
+ | font-family:Verdana, Arial, Helvetica, sans-serif; | ||
+ | } | ||
+ | |||
+ | #nav li { | ||
+ | float: left; | ||
+ | width: 149px; | ||
+ | margin-right: 41px; | ||
+ | } | ||
+ | |||
+ | #nav li ul { | ||
+ | position: absolute; | ||
+ | width: 130px; | ||
+ | left: -999em; | ||
+ | |||
+ | } | ||
+ | |||
+ | #nav li:hover ul { | ||
+ | left: auto; | ||
+ | text-decoration: none; | ||
+ | color: white !important; | ||
+ | |||
+ | } | ||
+ | |||
+ | #nav li:hover ul, #nav li.sfhover ul { | ||
+ | left: auto; | ||
+ | text-decoration: none; | ||
+ | color: white !important; | ||
+ | } | ||
+ | |||
+ | sfHover = function() { | ||
+ | var sfEls = document.getElementById("nav").getElementsByTagName("LI"); | ||
+ | for (var i=0; i<sfEls.length; i++) { | ||
+ | sfEls[i].onmouseover=function() { | ||
+ | this.className+=" sfhover"; | ||
+ | } | ||
+ | sfEls[i].onmouseout=function() { | ||
+ | this.className=this.className.replace(new RegExp(" sfhover\\b"), ""); | ||
+ | } | ||
+ | } | ||
+ | } | ||
+ | if (window.attachEvent) window.attachEvent("onload", sfHover); | ||
+ | |||
+ | |||
+ | body { | ||
+ | font: 10px normal Verdana, Arial, Helvetica, sans-serif; | ||
+ | |||
+ | padding: 0; | ||
+ | } | ||
+ | h1 { | ||
+ | font: 3em normal Verdana, 'Times New Roman', Times, serif; | ||
+ | color: #333; | ||
+ | margin: 0; | ||
+ | padding: 5px 0; | ||
+ | } | ||
+ | h3 { | ||
+ | font: 17px normal Verdana, 'Times New Roman', Times, serif; | ||
+ | color: #0000; | ||
+ | margin: 0; | ||
+ | padding: 5px 0; | ||
+ | margin: 10px; | ||
+ | text-align:justify; | ||
+ | margin-top: 8px; | ||
+ | margin-bottom: 20px; | ||
+ | margin-left: 20px; | ||
+ | margin-right: 20px; | ||
+ | } | ||
+ | h1 small{ | ||
+ | font: 0.2em normal Verdana, Arial, Helvetica, sans-serif; | ||
+ | text-transform:uppercase; | ||
+ | letter-spacing: 1.5em; | ||
+ | display: block; | ||
+ | color: #fff; | ||
+ | } | ||
+ | .container {width: 970px; margin: 0 auto;} | ||
+ | |||
+ | .curved { | ||
+ | -moz-border-radius: 10px; | ||
+ | -webkit-border-radius: 10px; | ||
+ | behavior:url(border-radius.htc); | ||
+ | } | ||
+ | |||
+ | |||
+ | |||
- | |||
+ | ul#topnav { | ||
+ | margin: 0; padding: 0; | ||
+ | float: left; | ||
+ | width: 970px; | ||
+ | list-style: none; | ||
+ | position: relative; | ||
+ | font-size: 18.8px; | ||
+ | background: url(topnav_stretch.gif) repeat-x; | ||
+ | } | ||
+ | ul#topnav li { | ||
+ | float: left; | ||
+ | margin: 0; padding: 0; | ||
+ | border-right: 1px solid #555; | ||
+ | } | ||
+ | ul#topnav li a { | ||
+ | padding: 10px 15px; | ||
+ | display: block; | ||
+ | color: darkblue; | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | li span { | ||
+ | float: left; | ||
+ | padding: 15px 0; | ||
+ | position: absolute; | ||
+ | left: 0; top:40px; | ||
+ | display: none; | ||
+ | width: 965px; | ||
+ | background: lightblue; | ||
+ | color: white; | ||
+ | -moz-border-radius-bottomright: 5px; | ||
+ | -khtml-border-radius-bottomright: 5px; | ||
+ | -webkit-border-bottom-right-radius: 5px; | ||
+ | -moz-border-radius-bottomleft: 5px; | ||
+ | -khtml-border-radius-bottomleft: 5px; | ||
+ | -webkit-border-bottom-left-radius: 5px; | ||
+ | } | ||
+ | ul#topnav li:hover span { display: block; } | ||
+ | ul#topnav li span a { display: inline; } | ||
+ | ul#topnav li span a:hover {text-decoration: underline;} | ||
+ | </style> | ||
+ | <script type="text/javascript" | ||
+ | src="http://ajax.googleapis.com/ajax/libs/jquery/1.3/jquery.min.js"></script> | ||
+ | <script type="text/javascript"> | ||
+ | $(document).ready(function() { | ||
+ | |||
+ | $("ul#topnav li").hover(function() { //Hover over event on list item | ||
+ | $(this).css({ 'background' : '#1376c9 url(topnav_active.gif) repeat-x'}); //Add background color + image on hovered list item | ||
+ | $(this).find("span").show(); //Show the subnav | ||
+ | } , function() { //on hover out... | ||
+ | $(this).css({ 'background' : 'none'}); //Ditch the background | ||
+ | $(this).find("span").hide(); //Hide the subnav | ||
+ | }); | ||
+ | |||
+ | }); | ||
+ | </script> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
+ | <div class=center> | ||
- | === | + | <div class=col_center> |
+ | <div class=col_center_top><b>BioBricks</b></div><!-- fin clase col_left_top--> | ||
+ | <div class=col_center_center> | ||
- | + | <br> | |
- | <groupparts> | + | <p>We have contributed to the Registry with three <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Valencia&Done=1" TARGET="_blank" title="Team:Valencia/Parts"> set of parts </a> of three different bacterian functions. We have worked with bacteriocins which are protein compounds produced by bacteria that inhibit or kill closed related species, like this we can get a good decontamination of water.</p> |
+ | <p>For this research we use, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K565001" TARGET="_blank"><b>COLICIN G</b></a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K565002" TARGET="_blank"><b>COLICIN H</b></a>.</p> | ||
+ | <p>We have used two different colicins, toxic exoproteins produced by Escherichia coli and related bacteria. To inhibit bacterial growth of strains similar or close, during the growth of their cultures. They are the best-characterized group of bacteriocins. Synthesis of colicins is coded by genes located on Col plasmids.</p> | ||
+ | <p>Their interaction with sensitive bacteria includes three steps: (1) binding of the colicin molecule to a specific receptor in the bacterial outer membrane; (2) its translocation through the cell envelope; and (3) its lethal interaction with the specific molecular target in the cell.</p> | ||
+ | <p>Afterwards we have utilized another bacteriocin too: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K565004" TARGET="_blank"><b>Microcin C51 (MccC51)</b></a>. </p> | ||
+ | <p>Microcins are gene-encoded antimicrobial polipeptides secreted by certains Enterobacteriaceae. It is an antimicrobial nucleotide-heptapeptide produced by a natural Escherichia coli strain. It is found in a plasmid, which carry the genes involved in MccC51 production, secretion, and self-immunity.</p> | ||
+ | <p>We wat to regulate these molecules' activity through different levels of pH. And we want <a href="https://2011.igem.org/Team:Valencia/Project2" TARGET="_blank"><i>Synecocystis</i> sp. PCC6803</a> to cause this pH change. We will prove how change this activity.</p> | ||
+ | |||
+ | </div><!-- fin clase col_center_top--> | ||
+ | <!-- fin clase col_left_top--> | ||
+ | </div><!-- fin clase col_center--> | ||
+ | </div><!-- fin clase center--> | ||
+ | </body></html> | ||
+ | <center><groupparts>iGEM011 Valencia</groupparts></center> |
Latest revision as of 02:42, 22 September 2011
We have contributed to the Registry with three set of parts of three different bacterian functions. We have worked with bacteriocins which are protein compounds produced by bacteria that inhibit or kill closed related species, like this we can get a good decontamination of water.
For this research we use, COLICIN G and COLICIN H.
We have used two different colicins, toxic exoproteins produced by Escherichia coli and related bacteria. To inhibit bacterial growth of strains similar or close, during the growth of their cultures. They are the best-characterized group of bacteriocins. Synthesis of colicins is coded by genes located on Col plasmids.
Their interaction with sensitive bacteria includes three steps: (1) binding of the colicin molecule to a specific receptor in the bacterial outer membrane; (2) its translocation through the cell envelope; and (3) its lethal interaction with the specific molecular target in the cell.
Afterwards we have utilized another bacteriocin too: Microcin C51 (MccC51).
Microcins are gene-encoded antimicrobial polipeptides secreted by certains Enterobacteriaceae. It is an antimicrobial nucleotide-heptapeptide produced by a natural Escherichia coli strain. It is found in a plasmid, which carry the genes involved in MccC51 production, secretion, and self-immunity.
We wat to regulate these molecules' activity through different levels of pH. And we want Synecocystis sp. PCC6803 to cause this pH change. We will prove how change this activity.