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Team:Tsinghua/Notebook/6 July 2011
From 2011.igem.org
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| - | [[pET15b OmpA-GFP construct]] | + | ==[[pET15b OmpA-GFP construct]]== |
Digest GFP vectors with NcoI (NEB) + CIP (NEB) 37 centigrade, 3hrs. | Digest GFP vectors with NcoI (NEB) + CIP (NEB) 37 centigrade, 3hrs. | ||
Recycle the vector via isopropanol precipitation. | Recycle the vector via isopropanol precipitation. | ||
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5ul vector + 2ul OmpA inserts + Takara solution I mix. RT 1h | 5ul vector + 2ul OmpA inserts + Takara solution I mix. RT 1h | ||
Transform DH5a with all the reaction mix, spread on LB plates + Ampicillin. | Transform DH5a with all the reaction mix, spread on LB plates + Ampicillin. | ||
| - | YX | + | YX |
Latest revision as of 14:41, 2 September 2011
pET15b OmpA-GFP construct
Digest GFP vectors with NcoI (NEB) + CIP (NEB) 37 centigrade, 3hrs. Recycle the vector via isopropanol precipitation. Ligation 5ul vector + 2ul OmpA inserts + Takara solution I mix. RT 1h Transform DH5a with all the reaction mix, spread on LB plates + Ampicillin. YX
