Team:Tokyo Metropolitan/Project

From 2011.igem.org

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== '''Overall project''' ==
 
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We propose the project "Bee.coli" as the micro injection system. <br />
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Bee.coli has the ability to move fast and target the pathogens concentrated area. <br />
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Getting close to the pathogens, it sends the killer gene to them with conjugation. Then the pathogens will be killed.<br />
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To accomplish this project, we planed to add three function to E.coli.<br />
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First, expression of the mutant HNS proteins enable E.coli to move 1.5 times faster. This contributes to increasing the frequency of conjugation.<br />
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<font size="6">Project "BeE.coli"<hr></font>
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Second function is new taxis for AHL. We make the target bacteria as the model of the pathogen, and they produce AHL.
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We propose the project "BeE.coli" as a micro injection system.BeE.coli has the abilities to move fast and target pathogens concentrated area.Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed.
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Bee.coli will often go for where high concentration of AHL. That is, where the target bacteria are concentrated. <br />
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</td><td width="200" align="center"><img src="https://static.igem.org/mediawiki/2011/c/c7/Tokyo_Metropolitan_BeEcoli100px.png"></td></tr></table>
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Last, the killer gene with the conjugative plasmid. Since Bee.coli also has the anti killer gene, it is alive and kill the pathogens with conjugation.<br />
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In the future, Bee.coli will help the medical or environmental scenes.
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== Project Details==
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<tr height="200"><td width="400"><img src="https://static.igem.org/mediawiki/2011/3/3e/Tokyo_Metropolitan_projectS.jpg" width="400"></td><td width="400">Speeding Up Device makes E.coli to move in higher speed than usual. E.coli have HNS protein gene in their genome. HNS is an Escherichia coli nucleoid protein known only to function as a modulator of gene expression. Gina M. Donato said that specific single amino acid substitutions in H-NS caused an approximately 50% increase in flagellum rotational speed. So we have E.coli to express HNS mutant T108I gene with plasmid.<br>
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<a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/SpeedUp">⇒Click to see more description.</a></td></tr>
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<tr height="200"><td width="400">Targeting Device gives new chemotaxis to E.coli. With this device, BeE.coli go for the target bacteria concentrated area. It is constructed to make chassis move in straight line when it receives a kind of chemicals. If the target bacteria produces a specific chemical substance, you can make chassis to swim for target bacteria by putting a appropriate promoter into this device.<br>
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<a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/Targeting">⇒Click to see more description.</a></td><td width="400">
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<img src="https://static.igem.org/mediawiki/2011/d/d2/Tokyo_Metropolitan_projectT.jpg" width="400"></td></tr>
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<td width="400"><img src="https://static.igem.org/mediawiki/2011/9/9d/Tokyo_Metropolitan_projectK.jpg" width="400"></td>
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<td width="400">When bee attack enemy, they sting needle and inject toxin. In Japan, the most cause of death from animal is attacted from bee (especially wasp).Bee is awful animal but taking a hint from it, we propose new system for killing bacteria using conjugation.<br>
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=== Part 2 ===
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<a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/Killing">⇒Click to see more description.</a><br>
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In addition, we propose a new assay of killing with conjugation.<br>
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<a href="https://2011.igem.org/Team:Tokyo_Metropolitan/Project/Visualization">⇒Click to see description.</a>
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
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Latest revision as of 08:50, 5 October 2011

Project "BeE.coli"
 We propose the project "BeE.coli" as a micro injection system.BeE.coli has the abilities to move fast and target pathogens concentrated area.Getting close to pathogens, it sends the killer gene to them with conjugation. Then pathogens will be killed.
Speeding Up Device makes E.coli to move in higher speed than usual. E.coli have HNS protein gene in their genome. HNS is an Escherichia coli nucleoid protein known only to function as a modulator of gene expression. Gina M. Donato said that specific single amino acid substitutions in H-NS caused an approximately 50% increase in flagellum rotational speed. So we have E.coli to express HNS mutant T108I gene with plasmid.
⇒Click to see more description.
Targeting Device gives new chemotaxis to E.coli. With this device, BeE.coli go for the target bacteria concentrated area. It is constructed to make chassis move in straight line when it receives a kind of chemicals. If the target bacteria produces a specific chemical substance, you can make chassis to swim for target bacteria by putting a appropriate promoter into this device.
⇒Click to see more description.
When bee attack enemy, they sting needle and inject toxin. In Japan, the most cause of death from animal is attacted from bee (especially wasp).Bee is awful animal but taking a hint from it, we propose new system for killing bacteria using conjugation.
⇒Click to see more description.
In addition, we propose a new assay of killing with conjugation.
⇒Click to see description.

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