Team:Bielefeld-Germany/HPLC isocratic
From 2011.igem.org
(Difference between revisions)
JSchwarzhans (Talk | contribs) |
JSchwarzhans (Talk | contribs) (→HPLC method) |
||
(3 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
===HPLC method=== | ===HPLC method=== | ||
* C18 reverse phase column | * C18 reverse phase column | ||
- | * Isocratic method: 45 % [[Team:Bielefeld-Germany/Protocols#Used_chemicals | | + | * Isocratic method: 45 % [[Team:Bielefeld-Germany/Protocols#Used_chemicals | Acetonitrile]] |
* Flow = 0.6 mL min<sup>-1</sup> | * Flow = 0.6 mL min<sup>-1</sup> | ||
* UV-detection at 227 nm | * UV-detection at 227 nm | ||
+ | * Internal standard: 100 mg L<sup>-1</sup> [[Team:Bielefeld-Germany/Protocols#Used_chemicals | Bisphenol F]] (BPF) | ||
* Column: | * Column: | ||
** Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer] | ** Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer] | ||
Line 20: | Line 21: | ||
** Series 1050 by [http://www.hp.com/ Hewlett Packard] | ** Series 1050 by [http://www.hp.com/ Hewlett Packard] | ||
- | [[File:Bielefeld2011 BPA + Abbauprodukt-2.JPG|900px | + | [[File:Bielefeld2011 BPA + Abbauprodukt-2.JPG|900px|left|thumb|Chromatogram obtained using the above described materials and methods. Three distinctive peaks are of interest: BPA at 7,3 min retention time, BPF at 5,8 min retention time and the degradation product of BPA at 4,6 min retention time.]] |
- | + | ||
- | + |
Latest revision as of 13:54, 31 August 2011
HPLC method
- C18 reverse phase column
- Isocratic method: 45 % Acetonitrile
- Flow = 0.6 mL min-1
- UV-detection at 227 nm
- Internal standard: 100 mg L-1 Bisphenol F (BPF)
- Column:
- Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer]
- Dimensions: 150 x 4.6 mm with precolumn
- Particle size: 5 µm
- Pore size: 100 Å
- Material: silica gel
- Software:
- Clarity (Version 3.0.5.505) by [http://www.dataapex.com/ Data Apex]
- Autosampler:
- Midas by [http://www.spark.nl/ Spark Holland]
- Tray cooling: 10 °C
- Pump:
- L-6200A Intelligent Pump by [http://www.hitachi.com/ Hitachi]
- UV-Detector:
- Series 1050 by [http://www.hp.com/ Hewlett Packard]