Team:UNICAMP-EMSE Brazil/protocols

From 2011.igem.org

(Difference between revisions)
(UNICAMP-EMSE team Protocols)
 
(One intermediate revision not shown)
Line 11: Line 11:
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/thermocompetent_prepare '''Preparation of Thermocompetent ''E. coli'' ''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/thermocompetent_prepare '''Preparation of Thermocompetent ''E. coli'' ''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_electro '''Transforming bacteria - Electroporation''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_electro '''Transforming bacteria - Electroporation''']
-
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_chimio '''Transforming bacteria - chimio-transformation''']
+
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_chemo '''Transforming bacteria - Chemo-transformation''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria '''Transforming bacteria - Heat Shock''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria '''Transforming bacteria - Heat Shock''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Mini_prep '''Miniprep''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Mini_prep '''Miniprep''']
Line 55: Line 55:
Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses"
Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses"
Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)"
Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)"
-
 
+
Q[21] = "<i> Relationship is not like those colorful things where everything fits perfectly ... The name of that is LEGO!</i> Izabella's Mom (mother of one of UNICAMP-EMSE team student)"
 +
Q[22] = "<i> Nice to meet you, I am Marianna</i> Marianna Favaro (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)"
 +
Q[23] = "<i> Who is this guy?</i> Danieli Gonçalves (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)"
 +
Q[24] = "<i> That guy is your friend?</i> Izabella Neshich (UNICAMP-EMSE team student, about Thibault, after he cut his hair and become unrecognizable)"
 +
Q[25] = "<i> But Nemaila said me that it was good!</i> Thibault Sabattier (UNICAMP-EMSE team student)"
 +
Q[26] = "<i> That guy is Caua Reymond</i> Marco Marinho (UNICAMP-EMSE team advisor said after Thibault cut his hair and become unrecognizable)"
 +
Q[27] = "<i> Marc don't put your head inside the flux or try to tan yourself in the UV transilluminator</i> Nemailla Bonturi (UNICAMP-EMSE team advisor warning the little student Marc Emery)"
 +
Q[28] = "<i> Piri-piri-piri-pi-pi-piri-pi (UNICAMP-EMSE team students Izabella, Danieli, Marianna, Juliana and advisor Nemailla singing a traditional song)"
 +
Q[29] = "<i> Everythings works at the end. If it has not worked, it's because we have not arrived to the end (UNICAMP-EMSE team students Izabella, Danieli, Marianna, Juliana and advisor Nemailla singing a traditional song)"
 +
Q[30] = "<i> That guy is your friend?</i> Izabella Neshich (UNICAMP-EMSE team student, about Thibault, after he cut his hair and become unrecognizable)"
 +
Q[31] = "<i> Who are you? What did you do with Thibault?</i> Juliana Canto (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)"
 +
Q[32] = "<i> When you are in hell, hugh satan </i> Nemailla Bonturi (UNICAMP-EMSE team advisor, explaining to Claire about the lab life infortunities)"

Latest revision as of 18:19, 28 September 2011

UNICAMP-EMSE team Protocols

Back to Notebook ↑

  1. Culture Media
  2. Preparation of Electrocompetent E. coli
  3. Preparation of Chemically competent E. coli
  4. Preparation of Thermocompetent E. coli
  5. Transforming bacteria - Electroporation
  6. Transforming bacteria - Chemo-transformation
  7. Transforming bacteria - Heat Shock
  8. Miniprep
  9. Restriction Enzyme Digestion
  10. DNA gel extraction
  11. Ligation