Team:UNICAMP-EMSE Brazil/protocols
From 2011.igem.org
(Difference between revisions)
Neshich.iap (Talk | contribs) (→UNICAMP-EMSE team Protocols) |
Neshich.iap (Talk | contribs) |
||
(4 intermediate revisions not shown) | |||
Line 7: | Line 7: | ||
<font size="3"> | <font size="3"> | ||
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Culture '''Culture Media'''] | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Culture '''Culture Media'''] | ||
- | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/electrocompetent_prepare '''Preparation of | + | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/electrocompetent_prepare '''Preparation of Electrocompetent ''E. coli'' '''] |
- | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/chimiocompetent_prepare '''Preparation of | + | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/chimiocompetent_prepare '''Preparation of Chemically competent ''E. coli'' '''] |
- | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/thermocompetent_prepare '''Preparation of | + | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/thermocompetent_prepare '''Preparation of Thermocompetent ''E. coli'' '''] |
- | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/ | + | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_electro '''Transforming bacteria - Electroporation'''] |
- | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/ | + | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_chemo '''Transforming bacteria - Chemo-transformation'''] |
- | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/ | + | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria '''Transforming bacteria - Heat Shock'''] |
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Mini_prep '''Miniprep'''] | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Mini_prep '''Miniprep'''] | ||
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Digestion '''Restriction Enzyme Digestion'''] | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Digestion '''Restriction Enzyme Digestion'''] | ||
Line 55: | Line 55: | ||
Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses" | Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses" | ||
Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)" | Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)" | ||
- | + | Q[21] = "<i> Relationship is not like those colorful things where everything fits perfectly ... The name of that is LEGO!</i> Izabella's Mom (mother of one of UNICAMP-EMSE team student)" | |
+ | Q[22] = "<i> Nice to meet you, I am Marianna</i> Marianna Favaro (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)" | ||
+ | Q[23] = "<i> Who is this guy?</i> Danieli Gonçalves (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)" | ||
+ | Q[24] = "<i> That guy is your friend?</i> Izabella Neshich (UNICAMP-EMSE team student, about Thibault, after he cut his hair and become unrecognizable)" | ||
+ | Q[25] = "<i> But Nemaila said me that it was good!</i> Thibault Sabattier (UNICAMP-EMSE team student)" | ||
+ | Q[26] = "<i> That guy is Caua Reymond</i> Marco Marinho (UNICAMP-EMSE team advisor said after Thibault cut his hair and become unrecognizable)" | ||
+ | Q[27] = "<i> Marc don't put your head inside the flux or try to tan yourself in the UV transilluminator</i> Nemailla Bonturi (UNICAMP-EMSE team advisor warning the little student Marc Emery)" | ||
+ | Q[28] = "<i> Piri-piri-piri-pi-pi-piri-pi (UNICAMP-EMSE team students Izabella, Danieli, Marianna, Juliana and advisor Nemailla singing a traditional song)" | ||
+ | Q[29] = "<i> Everythings works at the end. If it has not worked, it's because we have not arrived to the end (UNICAMP-EMSE team students Izabella, Danieli, Marianna, Juliana and advisor Nemailla singing a traditional song)" | ||
+ | Q[30] = "<i> That guy is your friend?</i> Izabella Neshich (UNICAMP-EMSE team student, about Thibault, after he cut his hair and become unrecognizable)" | ||
+ | Q[31] = "<i> Who are you? What did you do with Thibault?</i> Juliana Canto (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)" | ||
+ | Q[32] = "<i> When you are in hell, hugh satan </i> Nemailla Bonturi (UNICAMP-EMSE team advisor, explaining to Claire about the lab life infortunities)" | ||
Latest revision as of 18:19, 28 September 2011
Home | Project | Methods | Results | Data | Team | Notebook | Human Practices | Safety | Profile | Sponsors | Wix |
UNICAMP-EMSE team Protocols
Back to Notebook ↑
- Culture Media
- Preparation of Electrocompetent E. coli
- Preparation of Chemically competent E. coli
- Preparation of Thermocompetent E. coli
- Transforming bacteria - Electroporation
- Transforming bacteria - Chemo-transformation
- Transforming bacteria - Heat Shock
- Miniprep
- Restriction Enzyme Digestion
- DNA gel extraction
- Ligation