Team:UNICAMP-EMSE Brazil/protocols
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{{Template:UNICAMP-EMSE Brazil_Main_Menu}} | {{Template:UNICAMP-EMSE Brazil_Main_Menu}} | ||
- | <div style="text-align: right;"> | + | ==<font size="5" color=teal>UNICAMP-EMSE team Protocols== |
+ | </font><div style="text-align: right;"> | ||
<font size="4" color="red">Back to [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook Notebook ↑]</font> | <font size="4" color="red">Back to [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook Notebook ↑]</font> | ||
</div> | </div> | ||
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Culture '''Culture Media'''] | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Culture '''Culture Media'''] | ||
- | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria '''Transforming bacteria'''] | + | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/electrocompetent_prepare '''Preparation of Electrocompetent ''E. coli'' '''] |
+ | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/chimiocompetent_prepare '''Preparation of Chemically competent ''E. coli'' '''] | ||
+ | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/thermocompetent_prepare '''Preparation of Thermocompetent ''E. coli'' '''] | ||
+ | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_electro '''Transforming bacteria - Electroporation'''] | ||
+ | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_chemo '''Transforming bacteria - Chemo-transformation'''] | ||
+ | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria '''Transforming bacteria - Heat Shock'''] | ||
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Mini_prep '''Miniprep'''] | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Mini_prep '''Miniprep'''] | ||
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Digestion '''Restriction Enzyme Digestion'''] | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Digestion '''Restriction Enzyme Digestion'''] | ||
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/DNA_gel_extraction '''DNA gel extraction'''] | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/DNA_gel_extraction '''DNA gel extraction'''] | ||
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Ligation '''Ligation'''] | #[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Ligation '''Ligation'''] | ||
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+ | <html> | ||
+ | <head> | ||
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+ | |||
+ | function makeQuote() { | ||
+ | |||
+ | Q = new Array(); | ||
+ | |||
+ | |||
+ | Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)" | ||
+ | Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)" | ||
+ | Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
+ | Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
+ | Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)" | ||
+ | Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)" | ||
+ | Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)" | ||
+ | Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
+ | Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
+ | Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
+ | Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
+ | Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
+ | Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga" | ||
+ | Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor" | ||
+ | Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student" | ||
+ | Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair" | ||
+ | Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil" | ||
+ | Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress" | ||
+ | Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)" | ||
+ | Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses" | ||
+ | Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)" | ||
+ | Q[21] = "<i> Relationship is not like those colorful things where everything fits perfectly ... The name of that is LEGO!</i> Izabella's Mom (mother of one of UNICAMP-EMSE team student)" | ||
+ | Q[22] = "<i> Nice to meet you, I am Marianna</i> Marianna Favaro (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)" | ||
+ | Q[23] = "<i> Who is this guy?</i> Danieli Gonçalves (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)" | ||
+ | Q[24] = "<i> That guy is your friend?</i> Izabella Neshich (UNICAMP-EMSE team student, about Thibault, after he cut his hair and become unrecognizable)" | ||
+ | Q[25] = "<i> But Nemaila said me that it was good!</i> Thibault Sabattier (UNICAMP-EMSE team student)" | ||
+ | Q[26] = "<i> That guy is Caua Reymond</i> Marco Marinho (UNICAMP-EMSE team advisor said after Thibault cut his hair and become unrecognizable)" | ||
+ | Q[27] = "<i> Marc don't put your head inside the flux or try to tan yourself in the UV transilluminator</i> Nemailla Bonturi (UNICAMP-EMSE team advisor warning the little student Marc Emery)" | ||
+ | Q[28] = "<i> Piri-piri-piri-pi-pi-piri-pi (UNICAMP-EMSE team students Izabella, Danieli, Marianna, Juliana and advisor Nemailla singing a traditional song)" | ||
+ | Q[29] = "<i> Everythings works at the end. If it has not worked, it's because we have not arrived to the end (UNICAMP-EMSE team students Izabella, Danieli, Marianna, Juliana and advisor Nemailla singing a traditional song)" | ||
+ | Q[30] = "<i> That guy is your friend?</i> Izabella Neshich (UNICAMP-EMSE team student, about Thibault, after he cut his hair and become unrecognizable)" | ||
+ | Q[31] = "<i> Who are you? What did you do with Thibault?</i> Juliana Canto (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)" | ||
+ | Q[32] = "<i> When you are in hell, hugh satan </i> Nemailla Bonturi (UNICAMP-EMSE team advisor, explaining to Claire about the lab life infortunities)" | ||
+ | |||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | index = Math.floor(Math.random() * Q.length); | ||
+ | |||
+ | document.writeln(Q[index]); | ||
+ | |||
+ | } | ||
+ | |||
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+ | |||
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Latest revision as of 18:19, 28 September 2011
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UNICAMP-EMSE team Protocols
Back to Notebook ↑
- Culture Media
- Preparation of Electrocompetent E. coli
- Preparation of Chemically competent E. coli
- Preparation of Thermocompetent E. coli
- Transforming bacteria - Electroporation
- Transforming bacteria - Chemo-transformation
- Transforming bacteria - Heat Shock
- Miniprep
- Restriction Enzyme Digestion
- DNA gel extraction
- Ligation