Team:UNICAMP-EMSE Brazil/protocols

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==<font size="5" color=teal>UNICAMP-EMSE team Protocols==
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<font size="4" color="red">Back to [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook Notebook ↑]</font>
<font size="4" color="red">Back to [https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook Notebook ↑]</font>
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==<font size="5" color=teal>UNICAMP-EMSE team Protocols==
 
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Culture '''Culture Media''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Culture '''Culture Media''']
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria '''Transforming bacteria''']
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/electrocompetent_prepare '''Preparation of Electrocompetent ''E. coli'' ''']
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/chimiocompetent_prepare '''Preparation of Chemically competent ''E. coli'' ''']
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/thermocompetent_prepare '''Preparation of Thermocompetent ''E. coli'' ''']
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_electro '''Transforming bacteria - Electroporation''']
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria_chemo '''Transforming bacteria - Chemo-transformation''']
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Transforming_bacteria '''Transforming bacteria - Heat Shock''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Mini_prep '''Miniprep''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Mini_prep '''Miniprep''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Digestion '''Restriction Enzyme Digestion''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Digestion '''Restriction Enzyme Digestion''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/DNA_gel_extraction '''DNA gel extraction''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/DNA_gel_extraction '''DNA gel extraction''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Ligation '''Ligation''']
#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/Ligation '''Ligation''']
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#[https://2011.igem.org/Team:UNICAMP-EMSE_Brazil/protocols/electrocompetent_prepare '''Preparation of electrocompetent ''E. coli'' ''']
 
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Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)"
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Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)"
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Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)"
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Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)"
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Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)"
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Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)"
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Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)"
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Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)"
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Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga"
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Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor"
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Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student"
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Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair"
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Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil"
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Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress"
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Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)"
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Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses"
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Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)"
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Q[21] = "<i> Relationship is not like those colorful things where everything fits perfectly ... The name of that is LEGO!</i> Izabella's Mom (mother of one of UNICAMP-EMSE team student)"
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Q[22] = "<i> Nice to meet you, I am Marianna</i> Marianna Favaro (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)"
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Q[23] = "<i> Who is this guy?</i> Danieli Gonçalves (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)"
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Q[24] = "<i> That guy is your friend?</i> Izabella Neshich (UNICAMP-EMSE team student, about Thibault, after he cut his hair and become unrecognizable)"
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Q[25] = "<i> But Nemaila said me that it was good!</i> Thibault Sabattier (UNICAMP-EMSE team student)"
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Q[26] = "<i> That guy is Caua Reymond</i> Marco Marinho (UNICAMP-EMSE team advisor said after Thibault cut his hair and become unrecognizable)"
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Q[27] = "<i> Marc don't put your head inside the flux or try to tan yourself in the UV transilluminator</i> Nemailla Bonturi (UNICAMP-EMSE team advisor warning the little student Marc Emery)"
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Q[28] = "<i> Piri-piri-piri-pi-pi-piri-pi (UNICAMP-EMSE team students Izabella, Danieli, Marianna, Juliana and advisor Nemailla singing a traditional song)"
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Q[29] = "<i> Everythings works at the end. If it has not worked, it's because we have not arrived to the end (UNICAMP-EMSE team students Izabella, Danieli, Marianna, Juliana and advisor Nemailla singing a traditional song)"
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Q[30] = "<i> That guy is your friend?</i> Izabella Neshich (UNICAMP-EMSE team student, about Thibault, after he cut his hair and become unrecognizable)"
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Q[31] = "<i> Who are you? What did you do with Thibault?</i> Juliana Canto (UNICAMP-EMSE team student, said after Thibault cut his hair and become unrecognizable)"
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Q[32] = "<i> When you are in hell, hugh satan </i> Nemailla Bonturi (UNICAMP-EMSE team advisor, explaining to Claire about the lab life infortunities)"
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index = Math.floor(Math.random() * Q.length);
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document.writeln(Q[index]);
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}
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Latest revision as of 18:19, 28 September 2011

UNICAMP-EMSE team Protocols

Back to Notebook ↑

  1. Culture Media
  2. Preparation of Electrocompetent E. coli
  3. Preparation of Chemically competent E. coli
  4. Preparation of Thermocompetent E. coli
  5. Transforming bacteria - Electroporation
  6. Transforming bacteria - Chemo-transformation
  7. Transforming bacteria - Heat Shock
  8. Miniprep
  9. Restriction Enzyme Digestion
  10. DNA gel extraction
  11. Ligation