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Team:UNICAMP-EMSE Brazil/Notebook/20 August 2011
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==20 August 2011== | ==20 August 2011== | ||
===Everybody:=== | ===Everybody:=== | ||
| - | [[Image:UNICAMP-EMSE_Brazil_Gel_20_08_11.jpg|thumb|right|alt=Gel 1: flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D).<br>Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D)|Electrophoresis Gel, 20/08/2011.]] | + | [[Image:UNICAMP-EMSE_Brazil_Gel_20_08_11.jpg|thumb|right|265px|alt=Gel 1: flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D).<br> |
| - | '''Objective:''' Check | + | |
| + | Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D)|Electrophoresis Gel, 20/08/2011.]] | ||
| + | '''Objective:''' Check the size of the synthetic DNA sequences (that didn´t work on 19/08/2011). | ||
*Agarose: 0,8% | *Agarose: 0,8% | ||
*Ladder: 100-10.000 bp (Fermentas) | *Ladder: 100-10.000 bp (Fermentas) | ||
<br> | <br> | ||
| - | *'''Picture: Upper part:''' | + | *'''Picture legend: Upper part:''' |
#'''Gel 1:''' flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D) | #'''Gel 1:''' flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D) | ||
#'''Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D) | #'''Gel 2:''' / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D) | ||
| - | Nd: non digested | + | <div style="color:blue">'''Nd: non digested'''<br>'''D: digested''' |
| - | D: digested | + | |
| + | |||
| + | </div> | ||
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| + | |||
| + | |||
| + | <html> | ||
| + | <img src="https://static.igem.org/mediawiki/2011/c/cb/UNICAMP-EMSE_Brazil_Stresswars.png" width="893px"></img> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <head> | ||
| + | <script language='JavaScript' type='text/JavaScript'> | ||
| + | |||
| + | function makeQuote() { | ||
| + | |||
| + | Q = new Array(); | ||
| + | |||
| + | |||
| + | Q[0] = "<i>I’m Luke Skywalker, I’m here to rescue you.</i>Luke, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[1] = "<i>Watch your mouth kid, or you’ll find yourself floating home.</i>Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[2] = "<i>Evacuate in our moment of triumph? I think you overestimate their chances.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[3] = "<i>The Force is strong with this one.</i> Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[4] = "<i> I have you now!</i> Darth Vader, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[5] = "<i> Fear is the path to the dark side. Fear leads to anger, anger leads to hate, hate leads to suffering.</i> MsC Yoda, Star Wars Episode I: The Phantom Menace (1999)" | ||
| + | Q[6] = "<i> No blasters! No blasters!.</i> A bartender, Star Wars: Episode IV-A New Hope (1977)" | ||
| + | Q[7] = "<i> Go to the center of the gravity's pull, and find your planet you will...</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[8] = "<i> Clear, your mind must be if you are to discover the real villains behind the plot.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[9] = "<i> Much to learn, you still have.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[10] = "<i> Meditate on this, I will.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[11] = "<i> Use the Force, Luke.</i> M.Sc. Yoda, Star Wars Episode II: Attack of the Clones (2002)" | ||
| + | Q[12] = "<i> May the Force be with you.</i> Mentioned several times throughout the Star Wars saga" | ||
| + | Q[13] = "<i> I really need a Jedi Bacteria.</i> Iolanda Albuquerque, UNICAMP-EMSE team advisor" | ||
| + | Q[14] = "<i> Another splendid day in Brazil!!!</i> Marc Emery, UNICAMP-EMSE team student" | ||
| + | Q[15] = "<i> Dont' cut, let your hair volume expressing all their feelings...</i> Thibault Sabattier (UNICAMP-EMSE team student) about Nemailla's hair" | ||
| + | Q[16] = "<i> What a good life!!</i> Louise Marais (UNICAMP-EMSE team student) about living in Brazil" | ||
| + | Q[17] = "<i> Gosh!I will have nigthmares tonight! </i> Danieli Gonçalves (UNICAMP-EMSE team student) about Thibault wearing Marianna's dress" | ||
| + | Q[18] = "<i> I will revenge myself against you and Marc Emery!!!</i> Nemailla Bonturi (UNICAMP-EMSE team advisor)" | ||
| + | Q[19] = "<i> Yeah, those dresses deserve us!</i> Marc Emery (UNICAMP-EMSE team student) about Marianna's dresses" | ||
| + | Q[20] = "<i> I am really starting getting worried with you guys!!</i> Iolanda Albuquerque (UNICAMP-EMSE team advisor)" | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | index = Math.floor(Math.random() * Q.length); | ||
| + | |||
| + | document.writeln(Q[index]); | ||
| + | |||
| + | } | ||
| + | |||
| + | </script> | ||
| + | |||
| + | </head> | ||
| + | |||
| + | <style> | ||
| + | #newquote {color:gray; font-family:verdana; font-size:11px; text-align:right; padding:5px;} | ||
| + | |||
| + | #newquote i {display:block; color:black; font-family:georgia, trebuchet ms; font-size:13px;} | ||
| + | |||
| + | #newquote small {display:block; text-align:right; margin-top:10px;} | ||
| + | |||
| + | ]]></b:skin> | ||
| + | </style> | ||
| + | </html> | ||
| + | |||
| + | |||
| + | <html> | ||
| + | <head> | ||
| + | <div id='newquote'> | ||
| + | |||
| + | <script language="javascript"> | ||
| + | makeQuote(); | ||
| + | </script> | ||
</div> | </div> | ||
| + | </head> | ||
| + | </html> | ||
Latest revision as of 00:15, 30 August 2011
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20 August 2011
Everybody:
Objective: Check the size of the synthetic DNA sequences (that didn´t work on 19/08/2011).
- Agarose: 0,8%
- Ladder: 100-10.000 bp (Fermentas)
- Picture legend: Upper part:
- Gel 1: flHDC1 (nd)/ flHDC1 (d)/ flHDC2 (nd)/ flHDC2 (d)/RBSTolC3 (nd)/ RBSTolC3(d)/ RBSTolC4 (nd)/ RBSTolC4 (d)/RBSGFP1 (nd)/RBSGFP1 (D)/ RBSGFP 3(ND)/RBSGFP 3 (D)/ RBSQSEBRBSQSEC 3 (ND)/ RBSQSEBRBSQSEC 3 (D)
- Gel 2: / RBSQSEBRBSQSEC 4 (ND)/ RBSQSEBRBSQSEC 4 (D)/STRONG PROMOTOR 3 (ND)/ STRONG PROMOTOR 3 (D)/ STRONG PROMOTOR 4 (ND)/ STRONG PROMOTOR 4 (D)/ SOX S 3 (ND)/ SOX S 3 (D)/ SOX S 4 (ND)/SOX S 4 (D)
Nd: non digested
D: digested
D: digested
