Team:WITS-CSIR SA

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                <img src="https://static.igem.org/mediawiki/2011/6/63/Wits_Logo_Biotweet_Rearrange.gif" alt="Biotweet" height="60px"
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                 Our Project Concept            </div>
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                <p>
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To genetically reprogram the chemotactic behaviour of E. coli, through the use of synthetic riboswitches. This will enable bacteria to search a defined area for a particular ligand and return to a set location (the starting point), where they can report on their findings.
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As a proof of principle, we want to test the ability of the reprogrammed cells to locate atrazine/theophylline on a petri-dish, and return to the initial point of departure for reporting. To do this we have three constructs that will make the bacteria toggle between two states of chemotactic responsiveness to different substances.</p>
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                  <a href="#">
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                 <div data-dojo-type="dijit.TitlePane" data-dojo-props="title:'Biotweet: A riboswitch-controlled location-based networking framework'">
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                 <br />
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                  Brad explaining some concepts to the team
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<div data-dojo-type="dijit.TitlePane" data-dojo-props="title:'What is Biotweet?' ">
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                  <a href="#">click here to see the list</a>.
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<p>A defining characteristic of humanity is the constantly evolving sophistication with which human beings communicate and store information. Biologically, data communication networks exist too. The transfer of data occurs via signalling molecules incorporated into highly regulated and integrated networks.Our goal is to construct a bacterial communication network that will allow a group of engineered bacteria to transport messages in a directed manner, with the final aim of completing location-based functional tasks.We have focused on one aspect of this network: the directed transport of the message-carrying “communication modules” within our biological network. These communication modules will take the form of bacteria, which can move over physical distances carrying information. We have achieved this through engineering the exogenously controlled chemotactic behaviour of bacteria using synthetic riboswitches to regulate the expression of the CheZ protein required for bacterial motility.
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                  <strong>This</strong> is the same thing as the last...
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                  <strong>A board</strong> ... with writing.
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                  <strong>Reading </strong>something.
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<td valign="left"> <img src="https://static.igem.org/mediawiki/2011/8/8e/Frontpage_newbanner1.jpg" alt="biotweet" height="432" />
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                      This project could have important implications in the fields of medicine, mining and water treatment. One would be able to send a "bacterial-messenger" out to detect certain substances which can act as chemoattractants - such as disease biomarkers or elemental metals. Via chemotaxis, the messenger ''E.coli'' will be able to search an area and locate the source of the chemoattractant. It will then travel back to the starting point to report back on its findings. Based on the information provided by these "messenger bacteria" a probability density map can be generated, from which the location of the chemoattractant source can be determined. A potential application in diagnostics could be the non-invasive detection and localisation of cancerous cells in the colon, for example.              </div>
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<strong>Project</strong>
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<p class="slider-text">Biotweet is a biological networking framework. Our project page details all laboratory and modelling aspects of this project <a style="color:white;" href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Concept"><b>[Read more]</b></a></p>  
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                            Meet the team                         </div>
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                            The CSIR Wits South Africa team consists of six enthusiastic undergraduate students
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                  each having their own area of expertise. Four of the members are studying science
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                  and two are studying engineering at the University of the Witwatersrand. The biologists
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                  are from the schools of Molecular and Cell Biology and Molecular Medicine and Hematology.
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                  the other, chemical engineering. This team is a dynamic one where each team member
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<p class="slider-text">Our team consists of five enthusiastic undergraduate students each having their own area of expertise. Four of the members are studying science
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                  has something unique to offer to the competition.
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              and one is studying engineering at the University of the Witwatersrand. <a style="color:white;" href="https://2011.igem.org/Team:WITS-CSIR_SA/AboutUs/MeetTheTeam"><b>[Read more]</b></a></p>
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                            Software                          </div>
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<strong>Parts submitted</strong>
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<p class="slider-text">We submitted 16 parts to the registry. We also characterized a number of parts, including two theophylline riboswitches! <a style="color:white;" href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Parts"><b>[Read more]</b></a></p>  
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                            We created the following software during the competition.                           </div>
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<strong>Achievements</strong>
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<p class="slider-text">The team is proud to be a prize winner and one of the finalists from the European jamboree! For more details of our achievements in this competition <a style="color:white;" href="https://2011.igem.org/Team:WITS-CSIR_SA/Project/Achievements"><b>[click here]</b>!</a>
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Latest revision as of 20:45, 28 October 2011

Media:Example.ogg<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Biotweet

Biotweet iGem

A defining characteristic of humanity is the constantly evolving sophistication with which human beings communicate and store information. Biologically, data communication networks exist too. The transfer of data occurs via signalling molecules incorporated into highly regulated and integrated networks.Our goal is to construct a bacterial communication network that will allow a group of engineered bacteria to transport messages in a directed manner, with the final aim of completing location-based functional tasks.We have focused on one aspect of this network: the directed transport of the message-carrying “communication modules” within our biological network. These communication modules will take the form of bacteria, which can move over physical distances carrying information. We have achieved this through engineering the exogenously controlled chemotactic behaviour of bacteria using synthetic riboswitches to regulate the expression of the CheZ protein required for bacterial motility.

biotweet biotweet


  • Project Image
    Project

    Biotweet is a biological networking framework. Our project page details all laboratory and modelling aspects of this project [Read more]

  • Meet the team

    Our team consists of five enthusiastic undergraduate students each having their own area of expertise. Four of the members are studying science and one is studying engineering at the University of the Witwatersrand. [Read more]

  • Example Slide Image 3
    Parts submitted

    We submitted 16 parts to the registry. We also characterized a number of parts, including two theophylline riboswitches! [Read more]

  • Example Slide Image 4
    Achievements

    The team is proud to be a prize winner and one of the finalists from the European jamboree! For more details of our achievements in this competition [click here]!



Retrieved from "http://2011.igem.org/Team:WITS-CSIR_SA"