Team:UT Dallas/NotebookWeek6
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<li class = "active"><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li> | <li class = "active"><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li> | ||
<li><a href="https://2011.igem.org/Team:UT_Dallas/HumanPractices"><font size="3" face="verdana">Human Practices</a></font></li> | <li><a href="https://2011.igem.org/Team:UT_Dallas/HumanPractices"><font size="3" face="verdana">Human Practices</a></font></li> | ||
- | <li><a href="https://2011.igem.org/Team:UT_Dallas/ | + | <li><a href="https://2011.igem.org/Team:UT_Dallas/Safety"><font size="3" face="verdana">Safety</a></font></li> |
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<h2><span> Week 6</span></h2> | <h2><span> Week 6</span></h2> | ||
- | <p> <font size="3" face=" | + | <p><font size = "5" face= "arial" color = "#184D79"><b>August 15-</b></font> |
+ | <blockquote><li type = "circle">PCR of ptetR, RBS, LuxI, term<li type = "circle">Gel electrophoresis and purification<li type = "circle">Glycerol stock and miniprep <li type = "circle">Incubation in 3 mL LB can overnight at 37 degrees Celsius and 220 rpm<li type = "circle">Test digestion for 1 hour and 30 minutes at 37 degrees Celsius<li type = "circle">Gel Electrophoresis<li type = "circle">Test Digestion Observation: Lane 5 gave positive results for FGF-R: 2 bands observed at predicted bands size. Lane 4, 7, 9 were all negative bad results because EcoRI was used but only 1 band showed. This suggests that FGFR was not inserted. Lanes 10, 11, 12 were CheZ* and all matched negative control. Results are bad for CheZ*- will be regrown/miniprep/ligated.<li type = "circle">Incubation in 3 mL LB chlora overnight at 37 degrees Celsius and 220 rpm | ||
+ | </blockquote> | ||
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+ | <p><font size = "5" face= "arial" color = "#184D79"><b>August 16-</b></font> | ||
+ | <blockquote><li type = "circle">Digestion; Gel Electrophoresis and Purification<li type = "circle">Ligation (10 minutes at room temperature)<li type = "circle">Transformation (50 µL DH5α, 2 µL DNA, 37 degrees Celsius overnight, all carb)<li type = "circle">Re- incubating – taking 20 µL of yesterday’s incubations and placing them in 3 mL of LB and appropriate<li type = "circle">Transformation (50 µL DH5α, 2 µL DNA, 37 degrees Celsius overnight, all carb)</blockquote> | ||
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+ | <p><font size = "5" face= "arial" color = "#184D79"><b>August 17-</b></font> | ||
+ | <blockquote><li type = "circle">Glycerol stock ToxR and CheZ* colonies (2nd attempt)<li type = "circle">Miniprep<li type = "circle">no results on gel<li type = "circle">Incubate in 3 mL LB carb overnight at 37 degrees Celsius and 220 rpm<li type = "circle">Transformation (50 µL DH5α, 2 µL DNA, 37 degrees Celsius overnight, all carb)<li type = "circle">Incubation in 3 mL of LB chlora overnight at 37 degrees Celsius and 220 rpm</blockquote> | ||
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+ | <p><font size = "5" face= "arial" color = "#184D79"><b>August 18-</b></font> | ||
+ | <blockquote><li type = "circle">Glycerol Stock- 350 µL cells and 150 µL 50% glycerol<li type = "circle">Miniprep<li type = "circle">Test digestion<li type = "circle">Digestion, gel electrophoresis and purification<li type = "circle">Glycerol stock colonies 4 and 5 CheZ*<li type = "circle">Miniprep<li type = "circle">Test digestion and gel electrophoresis<li type = "circle">Ligation at 16 degrees Celsius overnight<li type = "circle">Incubation in 3 mL LB carb overnight at 37 degrees Celsius and 220 rpm<li type = "circle">Plate (507011) from Agar Stab</blockquote> | ||
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Latest revision as of 02:14, 3 September 2011