Team:Freiburg/Notebook/8 August

From 2011.igem.org

(Difference between revisions)
(→NAME OF YOUR EXPERIMENT: Cloning)
 
(8 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Freiburg/Templates/header}}
{{:Team:Freiburg/Templates/header}}
 +
<html>
 +
<div id="notebook-page-header">
 +
<div id="notebook-back" width="100px" >
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/7_August">Previous entry</a>
 +
</div>
 +
<div id="notebook-title">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 8 August </a>
 +
</div>
 +
<div id="notebook-next">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/9_August">Next entry</a>
 +
</div>
 +
</div>
 +
</html>
 +
==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
-
===Quickchange===
+
===1. Quickchange===
<br/>
<br/>
'''Investigator:Jakob'''
'''Investigator:Jakob'''
-
'''PCR'''
 
Line 72: Line 85:
-
'''Digest'''
+
===2. Digest with DpnI===
-
with DpnI
+
-
'''Transformation'''
+
* Digest of the quickchange above
 +
 
 +
===3. Transformation===
 +
<br/>
 +
 
 +
* Transformation from digest above
 +
 
 +
 
 +
*Note: I'm such a fool...forgot the gel...
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
Line 97: Line 117:
==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
-
===NAME OF YOUR EXPERIMENT===
+
===Lysis + RBS===
-
 
+
-
'''Investigators:NAME'''
+
 +
'''Investigators:Theo'''
 +
Sent samples for sequencing, Testdigestions of whole temperature sensitive cassette showed no correct inserts! (:()
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
Line 109: Line 129:
'''Investigators: Sophie'''<br/>
'''Investigators: Sophie'''<br/>
continue from experiment: miniprep (the, 8.8.)<br/>
continue from experiment: miniprep (the, 8.8.)<br/>
-
Project name: new 3A assembly with amp vector
+
Project name: new 3A assembly with amp vector<br/>
Parts: psB1A3 (Eco & Pst)<br/>
Parts: psB1A3 (Eco & Pst)<br/>
GFP-pbd ( Xba & Pst)<br/>
GFP-pbd ( Xba & Pst)<br/>

Latest revision as of 01:06, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

green light receptor

1. Quickchange


Investigator:Jakob


Name:

Jakob

Date:

08.08.2011

Continue from Experiment:
Project Name:

Quickchange CcaR and CcaS

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20
10µl 5x Phusion Buffer
2.5µl Primer fw Primer: P12(CcaR) and P14(CcaS
2.5µl Primer dw Primer: P13(CcaR) and P15(CcaS)
1µl dNTPs
1µl DNA-Template DNA: CcaR and CcaS
0.5 µl Phusion (add in the end)



2. Digest with DpnI

  • Digest of the quickchange above

3. Transformation


  • Transformation from digest above


  • Note: I'm such a fool...forgot the gel...

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

Lysis + RBS

Investigators:Theo

Sent samples for sequencing, Testdigestions of whole temperature sensitive cassette showed no correct inserts! (:()

Precipitator

NAME OF YOUR EXPERIMENT: Cloning

Investigators: Sophie
continue from experiment: miniprep (the, 8.8.)
Project name: new 3A assembly with amp vector
Parts: psB1A3 (Eco & Pst)
GFP-pbd ( Xba & Pst)
PR4 or PR6 (Eco & Spe)
Why? The last cloning of Theo and Jakob produced one clone with GFP-pbd inside but without PR. So I'm trying again to clone with two different PRs with different RBS strenghts.
Stored in: "Minipreps, verdaut"-box

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/8_August"