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Team:Freiburg/Notebook/8 August
From 2011.igem.org
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{{:Team:Freiburg/Templates/header}} | {{:Team:Freiburg/Templates/header}} | ||
| + | <html> | ||
| + | <div id="notebook-page-header"> | ||
| + | <div id="notebook-back" width="100px" > | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/7_August">Previous entry</a> | ||
| + | </div> | ||
| + | <div id="notebook-title"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 8 August </a> | ||
| + | </div> | ||
| + | <div id="notebook-next"> | ||
| + | <a href="https://2011.igem.org/Team:Freiburg/Notebook/9_August">Next entry</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </html> | ||
| + | |||
==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
| - | ===Quickchange=== | + | ===1. Quickchange=== |
<br/> | <br/> | ||
'''Investigator:Jakob''' | '''Investigator:Jakob''' | ||
| - | |||
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| colspan="2" style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name: | | colspan="2" style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name: | ||
| - | Quickchange CcaR | + | Quickchange CcaR and CcaS |
|} | |} | ||
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2.5µl | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2.5µl | ||
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer fw | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer fw | ||
| - | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer: P12 | + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer: P12(CcaR) and P14(CcaS |
|- | |- | ||
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2.5µl | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 2.5µl | ||
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer dw | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer dw | ||
| - | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer: P13 | + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Primer: P13(CcaR) and P15(CcaS) |
|- | |- | ||
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| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1µl | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 1µl | ||
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA-Template | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA-Template | ||
| - | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA: CcaR | + | | style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| DNA: CcaR and CcaS |
|- | |- | ||
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|} | |} | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | ===2. Digest with DpnI=== | ||
| + | |||
| + | * Digest of the quickchange above | ||
| + | |||
| + | ===3. Transformation=== | ||
| + | <br/> | ||
| + | |||
| + | * Transformation from digest above | ||
| + | |||
| + | |||
| + | *Note: I'm such a fool...forgot the gel... | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
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==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
| - | === | + | ===Lysis + RBS=== |
| - | + | ||
| - | + | ||
| + | '''Investigators:Theo''' | ||
| + | Sent samples for sequencing, Testdigestions of whole temperature sensitive cassette showed no correct inserts! (:() | ||
==<span style="color:grey;">Precipitator</span>== | ==<span style="color:grey;">Precipitator</span>== | ||
| - | ===NAME OF YOUR EXPERIMENT=== | + | ===NAME OF YOUR EXPERIMENT: Cloning=== |
| + | |||
| + | '''Investigators: Sophie'''<br/> | ||
| + | continue from experiment: miniprep (the, 8.8.)<br/> | ||
| + | Project name: new 3A assembly with amp vector<br/> | ||
| + | Parts: psB1A3 (Eco & Pst)<br/> | ||
| + | GFP-pbd ( Xba & Pst)<br/> | ||
| + | PR4 or PR6 (Eco & Spe)<br/> | ||
| + | Why? The last cloning of Theo and Jakob produced one clone with GFP-pbd inside but without PR. So I'm trying again to clone with two different PRs with different RBS strenghts.<br/> | ||
| + | Stored in: "Minipreps, verdaut"-box | ||
| - | |||
{{:Team:Freiburg/Templates/footer}} | {{:Team:Freiburg/Templates/footer}} | ||
Latest revision as of 01:06, 22 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
