User:Allancrossman

From 2011.igem.org

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Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
 
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==Personal TODO list==
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<p class="h1">Allan Crossman</p>
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* What is density of displayed INP?
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Allan Crossman, member of [[Team:Edinburgh|Edinburgh 2011]].
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* Plan for zipper system.
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* Plan for pIII beads reactor?
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* Can we suppress the hover-over thing for pages with no hard words?
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* [[Team:Edinburgh/Carotenoids]]
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* Add hints.
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==Notes to self==
==Notes to self==
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* We MUST have (somewhere) a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.
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* [[Judging]]
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* ''inaZ'' is [http://www.ncbi.nlm.nih.gov/nuccore/71553748?from=1850733&to=1855854 here], broken by an IS.
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* [https://igem.org/Results?year=2011 2011 full results]
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* The bglX part has a PstI site in it. Once fixed, the official part docs will need to be redone.
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* [http://mic.sgmjournals.org/content/142/7/1659.full.pdf+html bglX info]
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* [http://www.lifesci.dundee.ac.uk/groups/tracy_palmer/docs/signals.htm Substrates of TAT pathway in ''E. coli'']
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* [[User:Allancrossman/Gibson]] and [[User:Allancrossman/Gibson2]]
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* [https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS strengths from Warsaw 2010]
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* [http://www.ncbi.nlm.nih.gov/nuccore/AF130864.1 pG8SAET]
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* [http://partsregistry.org/Part:BBa_K415151:Experience BBa_K415151:Experience] - can this count for anything?
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* [[Special:Watchlist]]
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==Video game concept==
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You control a humble ribosome. You are powered by sugar. Outside the cell is cellobiose floating around. You can express B-glucosidase on the cell exterior to get more sugar. You can express other stuff. There's cellulose floating around too. You can express cellulases. There's phage. You can express proteases or somesuch?
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==Recombination avoidance test?==
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If INP works, and if we have the money, we could test whether our recombination avoidance strategy actually works, by getting a new version of BBa_K265008 synthesised with synonymous codons, and setting up a construct such that some reporter (e.g. LacZ, YFP, or whatever) would only be expressed if recombination occurred:
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: Promoter - RBS - INP1 - Terminator - Space - INP2 - Reporter
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See if there is any difference between this and:
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: Promoter - RBS - INP1 - Terminator - Space - INP1 - Reporter
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...when cloned into a RecA positive ''E. coli'' strain.
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==Lignin degradation==
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* Requires glucose oxidase to supply H2O2, a cofactor for other enzymes...
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==Stuff others are doing==
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** Lignin peroxidase
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** Manganaese peroxidase
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** Laccase?
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Some of these use flavin cofactors but it seems proteins using these can be successfully displayed using INP (Van Bloois ''et al'', 2009).
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* [https://2011.igem.org/Special:Contributions/Yazbo91 Contributions: Yassen]
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* [https://2011.igem.org/Special:Contributions/L.Kopec Contributions: Lukasz]
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* [https://2011.igem.org/Special:Contributions/mclee Contributions: Lee]
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* [https://2011.igem.org/Special:Contributions/sylvia.ispasani Contributions: Sylvia]
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* [https://2011.igem.org/Special:Contributions/Fionntom Contributions: Fionn]
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* [https://2011.igem.org/Special:Contributions/Di.L Contributions: Di]
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==References==
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==Competitors in our track==
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* Van Bloois E, Winter RT, Janssen DB, Fraaije MW (2009) [http://www.springerlink.com/content/d471504054865565/fulltext.pdf Export of functional ''Streptomyces coelicolor'' alditol oxidase to the periplasm or cell surface of ''Escherichia coli'' and its application in whole-cell biocatalysis]. ''Applied Microbiology and Biotechnology'' '''83''': 679-687 (doi: 10.1007/s00253-009-1904-0).
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: '''Progress to MIT:'''
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:: [[Team:Edinburgh]] (gold)
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:: [[Team:Johns_Hopkins]] (bronze)
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:: [[Team:UTP-Panama]] (bronze)
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:: [[Team:Washington]] (gold, Americas overall winner)
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:: [[Team:Yale]] (gold, Americas finalist)
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: '''Did not progress:'''
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:: [[Team:Alberta]]
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:: [[Team:Debrecen_Hungary]]
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:: [[Team:HIT-Harbin]]
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:: [[Team:Korea_U_Seoul]]
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:: [[Team:Nevada]]
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:: [[Team:Tec-Monterrey]]
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:: [[Team:Tianjin]]
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:: [[Team:UNAM-Genomics_Mexico]]
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:: [[Team:UST-Beijing]]
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:: [[Team:WashU]]
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Latest revision as of 18:38, 28 October 2011