Team:Tokyo Metropolitan/Parts

From 2011.igem.org

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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__NOTOC__{{:Team:Tokyo_Metropolitan/Header}}
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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<div style="width: 800px; margin-left: 70px; padding-top: 25px; padding-left: 20px;">
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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==Parts==
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<groupparts>iGEM011 Tokyo_Metropolitan</groupparts>
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== BBa_K543002 / HNS mutant T108I ==
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Specific single amino acid substitutions in HNS protein caused an approximately 50% increase in flagellum rotational speed.
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== BBa_K543012 / plux-GFP (AHL inducible) ==
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This device is useful for detective of AHL(3OC6HSL). Under existence of AHL, GFP is expressed.
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== BBa_K543015 / TetR generator (J23100-B0031-R0040-B0015) ==
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TetR generator (J23100-B0031-R0040-B0015).
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Repress expression of GFP by Anti-killing device
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We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. we confirmed Anti-killing Device represseｓ GFP expression weakly.
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Need more experience to get quantitative date.
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== BBa_K543000 / HNS protein (wild type) ==
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This is a wild type HNS protein coding part from E.coli K12 genome. HNS is an Escherichia coli nucleoid protein known only to function as a modulator of gene expression.
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== BBa_K543001 / HNS wild-type protein generator ==
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This is HNS wild-type protein generator part. Use for assay of HNS mutant T108I protein generator part.
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== BBa_K543003 / HNS mutant T108I protein generator ==
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HNS mutant T108I protein generator
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== BBa_K543010 / AHL inducible CheZ generator ==
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This device expresses LuxR constitutively and cheZ is expressed under existence of AHL.
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== BBa_K543011 / plux with LuxR generator ==
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Expression of devices under plux is induced by AHL-LuxR complex.
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== BBa_K543013 / cheZ generator (AHL inducible) without promoter ==
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cheZ generator (AHL inducible) without promoter.
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== BBa_K543016 / Bacteriophage Lysis Cassette S105, R, and Rz under Tet promoter (conjugative) ==
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Bacteriophage Lysis Cassette S105（Holin), R(Endolysin), and Rz under pTet.
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{|align="justify"
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Using this device with tetR generator, cell lysis is repressed. But adding aTc, cell lysis is induced.
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|You can write a background of your team here. Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Tokyo_Metropolitan_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Tokyo_Metropolitan_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Tokyo_Metropolitan | Team Example]]
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|}
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<!--- The Mission, Experiments --->
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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== BBa_K543020 / OriTr-GFP ==
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!align="center"|[[Team:Tokyo_Metropolitan|Home]]
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!align="center"|[[Team:Tokyo_Metropolitan/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Tokyo_Metropolitan Official Team Profile]
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!align="center"|[[Team:Tokyo_Metropolitan/Project|Project]]
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!align="center"|[[Team:Tokyo_Metropolitan/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Tokyo_Metropolitan/Modeling|Modeling]]
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!align="center"|[[Team:Tokyo_Metropolitan/Notebook|Notebook]]
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!align="center"|[[Team:Tokyo_Metropolitan/Safety|Safety]]
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!align="center"|[[Team:Tokyo_Metropolitan/Attributions|Attributions]]
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|}
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OriTr(BBa_J01003)+GFP(BBa_I13504).
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== BBa_K543030 / [I714030][J23066][I714030] (Constitutive expression) ==
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===Parts===
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This parts is [I714030][J23066][I714030] under Constitutive promoter J23100.
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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== BBa_K543032 / assay system of killing by conjugation ==
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<groupparts>iGEM010 Tokyo_Metropolitan</groupparts>
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This part assays conjugation to second round of conjugation. Especially, it is useful to assay killing device by conjugation.

Latest revision as of 17:59, 5 October 2011

Parts

<groupparts>iGEM011 Tokyo_Metropolitan</groupparts>


BBa_K543002 / HNS mutant T108I

Specific single amino acid substitutions in HNS protein caused an approximately 50% increase in flagellum rotational speed.


BBa_K543012 / plux-GFP (AHL inducible)

This device is useful for detective of AHL(3OC6HSL). Under existence of AHL, GFP is expressed.


BBa_K543015 / TetR generator (J23100-B0031-R0040-B0015)

TetR generator (J23100-B0031-R0040-B0015).


Repress expression of GFP by Anti-killing device

We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. we confirmed Anti-killing Device represseｓ GFP expression weakly.

Need more experience to get quantitative date.


BBa_K543000 / HNS protein (wild type)

This is a wild type HNS protein coding part from E.coli K12 genome. HNS is an Escherichia coli nucleoid protein known only to function as a modulator of gene expression.


BBa_K543001 / HNS wild-type protein generator

This is HNS wild-type protein generator part. Use for assay of HNS mutant T108I protein generator part.


BBa_K543003 / HNS mutant T108I protein generator

HNS mutant T108I protein generator


BBa_K543010 / AHL inducible CheZ generator

This device expresses LuxR constitutively and cheZ is expressed under existence of AHL.


BBa_K543011 / plux with LuxR generator

Expression of devices under plux is induced by AHL-LuxR complex.


BBa_K543013 / cheZ generator (AHL inducible) without promoter

cheZ generator (AHL inducible) without promoter.


BBa_K543016 / Bacteriophage Lysis Cassette S105, R, and Rz under Tet promoter (conjugative)

Bacteriophage Lysis Cassette S105（Holin), R(Endolysin), and Rz under pTet.

Using this device with tetR generator, cell lysis is repressed. But adding aTc, cell lysis is induced.


BBa_K543020 / OriTr-GFP

OriTr(BBa_J01003)+GFP(BBa_I13504).

BBa_K543030 / [I714030][J23066][I714030] (Constitutive expression)

This parts is [I714030][J23066][I714030] under Constitutive promoter J23100.

BBa_K543032 / assay system of killing by conjugation

This part assays conjugation to second round of conjugation. Especially, it is useful to assay killing device by conjugation.

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Parts"